Acute metabolic response of Portunus trituberculatus to Vibrio alginolyticus infection

Acute metabolic response of Portunus trituberculatus to Vibrio alginolyticus infection
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三疣梭子蟹对溶藻弧菌感染的急性代谢反应

DOI:
10.1016/j.aquaculture.2016.05.041
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发表时间:
2016-10
期刊:
影响因子:
4.5
通讯作者:
Wang CL
Wang CL
中科院分区:
农林科学1区
文献类型:
--
作者:
Ye Yangfang;Xia Mengjie;Mu Changkao;Li Ronghua;Wang Chunlin;Ye Yangfang;Wang CL

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溶藻弧菌是引起三疣梭子蟹弧菌病的常见病原。梭子蟹对病毒代谢反应机制的研究。溶藻菌是进一步控制这种疾病所必需的。本文研究了梭子蟹鳃、肝胰腺和肌肉对急性V.使用1H NMR光谱结合多变量数据分析测定溶藻菌感染。结果表明,V.溶藻菌感染24 h后,蟹肌肉和鳃的代谢组发生显著变化。溶藻弧菌感染梭子蟹后,肌肉中氨基酸的合成受到抑制,能量积累得到改善,细胞免疫反应增强。这些代谢变化突出表现在氨基酸和肌苷酸的消耗以及肌肉中ATP和甜菜碱的积累。弧菌感染还引起鳃能量紊乱,表现为葡萄糖的消耗以及乳酸和腺苷酸的积累。这些结果提供了梭子蟹急性代谢组学变化的细节后V。本研究通过对溶藻弧菌感染的代谢产物的研究,揭示了其作为弧菌感染生物标志物的潜力,为螃蟹弧菌感染提供了潜在的代谢物生物标志物。
Vibrio alginolyticusis a common pathogen that causes vibrio disease in swimming crab,Portunus trituberculatus. An understanding of the metabolic response mechanism of swimming crab toV. alginolyticusis essential for further control of this disease. In the present study, we analyzed the metabolic responses of gill, hepatopancreas, and muscle of swimming crab to an acuteV. alginolyticusinfection using1H NMR spectroscopy coupled with multivariate data analysis. Our results showed thatV. alginolyticusinfection induced significant metabolomic alterations in crab muscle and gill within 24 h. These alterations showed tissue-specific in the infected swimming crabs.V. alginolyticusinfection resulted in depression of gluconeogenesis from amino acids, improvement of energy accumulation, and enhancement of cellular immune response of muscle. These metabolic alterations were highlighted in the depletion of amino acids and inosine monophosphate as well as in the accumulation of ATP and betaine in muscle. Vibrio infection also caused energy disturbance in gill, which manifested in the depletion of glucose and in the accumulation of lactate and adenosine monophosphate. These findings provided details of acute metabolomic alterations of swimming crab afterV. alginolyticusinfection and demonstrated the potentiality of altered metabolites as biomarker in vibrio infection.Statement of relevanceProvide potential metabolite biomarkers for vibrio infection of crab.
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