Postnatal characterization of cells in the accessory olfactory bulb of wild type and reeler mice.
Postnatal characterization of cells in the accessory olfactory bulb of wild type and reeler mice.
复制标题
DOI：
10.3389/fnana.2012.00015
复制
发表时间：
2012
影响因子：
2.9
通讯作者：
López-Mascaraque L
中科院分区：
文献类型：
--
作者：
Martín-López E;Corona R;López-Mascaraque L
嗅觉是啮齿类动物最重要的化学感觉。一般气味主要由主嗅觉系统检测，而大多数信息素信号由辅助嗅觉系统接收。大脑中的第一个中继发生在嗅球中，嗅球又细分为主嗅球和副嗅球（MOB/AOB）。鉴于AOB和MOB之间的细胞生成时间不同，并且AOB的细胞表征仍然有限，本工作的目标是首先定义AOB/MOB的分层，其次确定在对应于出生后早期发育的时间窗内AOB中的细胞表型。此外，由于reelin（Reln）缺乏与嗅觉学习缺陷有关，我们分析了reeler小鼠。首先，我们比较了AOB和MOB在早期胚胎阶段的分层。然后，使用特定的神经元和神经胶质标记物以及Reln衔接蛋白Dab 1建立细胞表型，以分析两种遗传背景的差异。AOB和MOB之间或野生型（wt）和reeler动物之间的细胞表型没有明显差异。然而，观察到相对于野生型小鼠的卷轴的颗粒细胞层的破坏。总之，在Reln缺陷小鼠中的AOB显示出相似的神经元和胶质细胞类型，仅颗粒神经元的组织受到影响。
Olfaction is the most relevant chemosensory sense of the rodents. General odors are primarily detected by the main olfactory system while most pheromonal signals are received by the accessory olfactory system. The first relay in the brain occurs in the olfactory bulb, which is subdivided in the main and accessory olfactory bulb (MOB/AOB). Given that the cell generation time is different between AOB and MOB, and the cell characterization of AOB remains limited, the goal of this work was first, the definition of the layering of AOB/MOB and second, the determination of cellular phenotypes in the AOB in a time window corresponding to the early postnatal development. Moreover, since reelin (Reln) deficiency has been related to olfactory learning deficits, we analyzed reeler mice. First, we compared the layering between AOB and MOB at early embryonic stages. Then, cell phenotypes were established using specific neuronal and glial markers as well as the Reln adaptor protein Dab1 to analyse differences in both genetic backgrounds. There was no apparent difference in the cell phenotypes among AOB and MOB or between wild type (wt) and reeler animals. However, a disruption in the granular cell layer of reeler with respect to wt mice was observed. In conclusion, the AOB in Reln-deficient mice showed similar neuronal and glial cell types being only affected the organization of granular neurons.
登录
查看更多内容
影响因子：
2.9
作者：
Kosaka, Toshio;Kosaka, Katsuko
通讯作者：
Kosaka, Katsuko
影响因子：
3.7
作者：
Martín-López E;Blanchart A;De Carlos JA;López-Mascaraque L
通讯作者：
López-Mascaraque L
影响因子：
3.5
作者：
Restrepo, D;Arellano, J;Lin, WH
通讯作者：
Lin, WH
影响因子：
2.5
作者：
HINDS, JW
通讯作者：
HINDS, JW
影响因子：
25
作者：
Hack, I;Bancila, M;Cremer, H
通讯作者：
Cremer, H