Hex1-related transcriptome of Trichoderma atroviride reveals expression patterns of ABC transporters associated with tolerance to dichlorvos
Hex1-related transcriptome of Trichoderma atroviride reveals expression patterns of ABC transporters associated with tolerance to dichlorvos
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深绿木霉 Hex1 相关转录组揭示了与敌敌畏耐受相关的 ABC 转运蛋白的表达模式
DOI：
10.1007/s10529-015-1806-4
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Jie Chen
中科院分区：
文献类型：
--
作者：
Jianan Sun;Chuanjin Yu;Zhicheng Liu;Jie Chen
目的：木霉对有机磷农药敌敌畏的耐受性是其在生物修复和害虫综合治理中应用的关键，但与耐受性相关的分子事件尚未阐明。结果：通过对野生型深绿木霉T23和敌敌畏胁迫下hex 1缺失突变株的RNA-seq分析，寻找与耐受性相关的基因。共鉴定出5382个差异表达基因，突出了T.阿维菌素对敌敌畏胁迫的响应。137个基因受到敌敌畏和hex 1的调控，编码主要的易化超家族、细胞色素P450、谷胱甘肽-S-转移酶、黄素蛋白、Hsp 70、Hsp 90等。暴露于敌敌畏2，6和24 h后，通过qRT-PCR确认7个选定的ABC转运蛋白基因的表达模式。结论：本研究提供了深入了解敌敌畏耐受木霉的遗传基础，可用于进一步开发生物修复或生物防治剂。
Objectives:The tolerance to organophosphate pesticide, dichlorvos, is essential for the application of Trichoderma in bioremediation and integrated pest management, although the molecular events associated with the tolerance process have not yet been elucidated.Results:RNA-seq analysis of wild-type Trichoderma atroviride T23 and the hex1-deleted mutant under dichlorvos stress was designed to search for genes involved in the tolerance process. A total of 5382 differentially expressed genes were identified, highlighting the complex transcriptional changes of T. atroviride in response to dichlorvos stress. 137 genes were regulated by dichlorvos and hex1, encoding major facilitator superfamilies, cytochrome P450, glutathione-S-transferase, flavoprotein, Hsp70, Hsp90, etc. Pathway and expression analysis indicated that ABC transporters were affected by the disruption of hex1 gene and might play a vital role in the tolerance process. Expression patterns of seven selected ABC transporter genes were confirmed by qRT-PCR after exposure to dichlorvos for 2, 6 and 24 h.Conclusions:The present study provides insights into the genetic basis of dichlorvos tolerance in Trichoderma that may be exploited for further development of bioremediation or biocontrol agents.
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