Single-Cell RNA Sequencing for Plant Research: Insights and Possible Benefits.

Single-Cell RNA Sequencing for Plant Research: Insights and Possible Benefits.
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植物研究的单细胞RNA测序:见解和可能的好处。

DOI:
10.3390/ijms23094497
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发表时间:
2022-04-19
影响因子:
5.6
通讯作者:
--
中科院分区:
生物学2区
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--
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近年来,单细胞RNA测序(scRNA-seq)技术的进步通过将分析的时空分辨率提高到单细胞分辨率,不断改变我们对生物系统的看法。将scRNA-seq应用于植物,可以全面表征常见和罕见的细胞类型和细胞状态,揭示新的细胞类型,揭示细胞类型之间的空间和发育关系。本文综述了scRNA-seq方法,重点介绍了scRNA-seq在植物科学中的应用,说明了为什么scRNA-seq是测序的主要参与者,并解释了单细胞转录组学技术在环境胁迫适应中的作用,以及单细胞转录组学的挑战和前景。总之,我们提出了单细胞测序在植物研究中的核心作用。
In recent years, advances in single-cell RNA sequencing (scRNA-seq) technologies have continued to change our views on biological systems by increasing the spatiotemporal resolution of our analysis to single-cell resolution. Application of scRNA-seq to plants enables the comprehensive characterization of both common and rare cell types and cell states, uncovering new cell types and revealing how cell types relate to each other spatially and developmentally. This review provides an overview of scRNA-seq methodologies, highlights the application of scRNA-seq in plant science, justifies why scRNA-seq is a master player of sequencing, and explains the role of single-cell transcriptomics technologies in environmental stress adaptation, alongside the challenges and prospects of single-cell transcriptomics. Collectively, we put forward a central role of single-cell sequencing in plant research.
从单细胞基因表达谱的细胞身份定量。
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