An odorant receptor from the southern house mosquito Culex pipiens quinquefasciatus sensitive to oviposition attractants.
An odorant receptor from the southern house mosquito Culex pipiens quinquefasciatus sensitive to oviposition attractants.
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DOI：
10.1371/journal.pone.0010090
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发表时间：
2010-04-08
期刊：
影响因子：
3.7
通讯作者：
Leal WS
中科院分区：
文献类型：
--
作者：
Pelletier J;Hughes DT;Luetje CW;Leal WS
昆虫气味受体（OR）是由与一个保守共受体相关的高度可变的气味结合亚基组成的异聚体。它们是开发新型蚊子引诱剂和驱虫剂的潜在分子靶标。已在疟疾蚊子（冈比亚按蚊）和黄热病蚊子（埃及伊蚊）中发现了 OR。然而，在南方家蚊库蚊（Culex quinquefasciatus）中，它们仍然未知，这种蚊子传播导致世界各地人类疾病的病原体，包括美国的西尼罗河病毒。我们结合了生物信息学、分子克隆和电生理学方法来识别和表征 Cx 中 OR 的反应概况。致倦五线虫。首先，我们揭示了该物种中由 158 个推定 OR 组成的大型多基因家族，其中包括三个蚊子物种中保守 OR 的亚组。使用非洲爪蟾卵母细胞表达系统，我们确定了 CquiOR2（一种触角特异性 OR）的响应特征，它与冈比亚按蚊 (AgamOR2) 和埃及伊蚊 (AaegOR2) 中推定的直系同源物具有高度同一性。我们发现 CquiOR2 对吲哚高度敏感，吲哚是 Cx 的产卵引诱剂。致倦五线虫。非洲爪蟾卵母细胞中表达的 CquiOR2 的反应概况类似于 Cx 触角短钝尖感器 (A2) 中的嗅觉受体神经元的反应概况。 quinquefasciatus，是产卵引诱剂的天然探测器。因此，第一个库蚊或去孤儿化是筛选产卵引诱剂的潜在分子靶点。
Insect odorant receptors (ORs) are heteromers comprised of highly variable odorant-binding subunits associated with one conserved co-receptor. They are potential molecular targets for the development of novel mosquito attractants and repellents. ORs have been identified in the malaria mosquito, Anopheles gambiae, and in the yellow fever mosquito, Aedes aegypti. However, they are still unknown in the Southern house mosquito, Culex quinquefasciatus, which transmits pathogens that cause human diseases throughout the world, including West Nile Virus in the United States. We have employed a combination of bioinformatics, molecular cloning and electrophysiology approaches to identify and characterize the response profile of an OR in Cx. quinquefasciatus. First, we have unveiled a large multigenic family of one-hundred-fifty-eight putative ORs in this species, including a subgroup of conserved ORs in three mosquito species. Using the Xenopus oocytes expression system, we have determined the response profile of CquiOR2, an antennae-specific OR, which shares high identity with putative orthologs in Anopheles gambiae (AgamOR2) and Aedes aegypti (AaegOR2). We show that CquiOR2 is highly sensitive to indole, an oviposition attractant for Cx. quinquefasciatus. The response profile of CquiOR2 expressed in Xenopus oocytes resembles that of an olfactory receptor neuron housed in the antennal short blunt-tipped sensilla (A2) of Cx. quinquefasciatus, which are natural detectors for oviposition attractants. This first Culex OR de-orphanized is, therefore, a potential molecular target for screening oviposition attractants.
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影响因子：
3.8
作者：
Smart, Renee;Kiely, Aidan;Warr, Coral G.
通讯作者：
Warr, Coral G.
影响因子：
2.1
作者：
Du, YJ;Millar, JG
通讯作者：
Millar, JG
影响因子：
2.6
作者：
Bohbot, J.;Pitts, R. J.;Zwiebel, L. J.
通讯作者：
Zwiebel, L. J.
影响因子：
3.7
作者：
Leal WS;Barbosa RM;Xu W;Ishida Y;Syed Z;Latte N;Chen AM;Morgan TI;Cornel AJ;Furtado A
通讯作者：
Furtado A
DOI：
10.1139/z70-048
发表时间：
1970-01-01
期刊：
CANADIAN JOURNAL OF ZOOLOGY - BACK YEAR PROJECT
影响因子：
--
作者：
MCIVER, S;CHARLTON, C
通讯作者：
CHARLTON, C