Identification and evaluation of ω-3 fatty acid desaturase genes for hyperfortifying α-linolenic acid in transgenic rice seed.
Identification and evaluation of ω-3 fatty acid desaturase genes for hyperfortifying α-linolenic acid in transgenic rice seed.
复制标题
DOI：
10.1093/jxb/ers051
复制
发表时间：
2012-05
影响因子：
6.9
通讯作者：
Qu le Q
中科院分区：
文献类型：
--
作者：
Liu HL;Yin ZJ;Xiao L;Xu YN;Qu le Q
α-亚麻酸 (ALA) 缺乏和饮食中 ω6:ω3 脂肪酸比例失衡是心血管疾病和炎症/自身免疫性疾病流行的主要原因。需要提高ALA含量并降低亚油酸(LA)与ALA的比例。从水稻和大豆中克隆了六个 ω-3 (Δ-15) 脂肪酸去饱和酶 (FAD) 基因。鉴定了蛋白质的亚细胞定位。 FAD 基因在胚乳特异性启动子、GluC 或 Ubi-1 启动子的控制下引入水稻，以评估其增加种子中 ALA 含量的潜力。内质网（ER）定位的GmFAD3-1和OsFAD3过表达株系种子中的ALA含量分别从0.36 mg g−1增加到8.57 mg g−1和10.06 mg g−1，比非转化子高23.8倍和27.9倍。高ALA含量的性状可稳定遗传3代。同源 OsFAD3 在催化水稻种子中 LA 转化为 ALA 方面比 GmFAD3-1 更活跃。内质网定位的GmFAD3-2/3和叶绿体定位的OsFAD7/8的过表达对增加水稻种子中ALA含量的作用较小。在该实验系统中，GluC启动子与Ubi-1相比具有优势。增强的 ALA 优先位于三酰甘油中的 sn-2 位置。一份高 ALA 米饭的份量可满足成人每日 ALA 需求量的 80% 以上。富含 ALA 的大米有望改善全球饮食中 ALA 缺乏的情况。
α-Linolenic acid (ALA) deficiency and a skewed of ω6:ω3 fatty acid ratio in the diet are a major explanation for the prevalence of cardiovascular diseases and inflammatory/autoimmune diseases. There is a need to enhance the ALA content and to reduce the ratio of linoleic acid (LA) to ALA. Six ω-3 (Δ-15) fatty acid desaturase (FAD) genes were cloned from rice and soybean. The subcellular localizations of the proteins were identified. The FAD genes were introduced into rice under the control of an endosperm-specific promoter, GluC, or a Ubi-1 promoter to evaluate their potential in increasing the ALA content in seeds. The ALA contents in the seeds of endoplasmic reticulum (ER)-localized GmFAD3-1 and OsFAD3 overexpression lines increased from 0.36 mg g−1 to 8.57 mg g−1and 10.06 mg g−1, respectively, which was 23.8- and 27.9-fold higher than that of non-transformants. The trait of high ALA content was stably inheritable over three generations. Homologous OsFAD3 is more active than GmFAD3-1 in catalysing LA conversion to ALA in rice seeds. Overexpression of ER-localized GmFAD3-2/3 and chloroplast-localized OsFAD7/8 had less effect on increasing the ALA content in rice seeds. The GluC promoter is advantageous compared with Ubi-1 in this experimental system. The enhanced ALA was preferentially located at the sn-2 position in triacylglycerols. A meal-size portion of high ALA rice would meet >80% of the daily adult ALA requirement. The ALA-rich rice could be expected to ameliorate much of the global dietary ALA deficiency.
登录
查看更多内容
影响因子：
1.9
作者：
Simopoulos, AP
通讯作者：
Simopoulos, AP
DOI：
10.1073/pnas.0511079103
发表时间：
2006-06-20
影响因子：
11.1
作者：
Damude, Howard G.;Zhang, Hongxiang;Yadav, Narendra S.
通讯作者：
Yadav, Narendra S.
影响因子：
4.3
作者：
Qu, LQ;Yoshihara, T;Takaiwa, F
通讯作者：
Takaiwa, F
影响因子：
6.2
作者：
Anai, T;Koga, M;Takagi, Y
通讯作者：
Takagi, Y
转基因水稻中六个麸质蛋白基因启动子的表达模式和活性。
影响因子：
6.9
作者：
Qu, Le Qing;Xing, Yan Ping;Liu, Wen Xian;Xu, Xiu Ping;Song, Yan Ru
通讯作者：
Song, Yan Ru