Improving Signal to Noise Ratios in Ion Mobility Spectrometry and Structures for Lossless Ion Manipulations (SLIM) using a High Dynamic Range Analog-to-Digital Converter.
Improving Signal to Noise Ratios in Ion Mobility Spectrometry and Structures for Lossless Ion Manipulations (SLIM) using a High Dynamic Range Analog-to-Digital Converter.
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DOI：
10.1021/jasms.1c00226
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发表时间：
2021-11-03
影响因子：
3.2
通讯作者：
Ibrahim YM
中科院分区：
文献类型：
--
作者：
Hollerbach AL;Giberson CM;Lee JY;Huntley AP;Smith RD;Ibrahim YM
信号数字化是离子迁移质谱（IMS-MS）工作流程中经常被忽视的部分，但它极大地影响了信噪比和MS分辨率测量。在这里，我们报告了一个2 GS/s，14位ADC与无损离子操作（SLIM-IMS-MS）结构的集成，并将其性能与常用的8位ADC进行了比较。14位ADC将数字化噪声降低了约6倍，这在很大程度上是由于使用了更小的位大小。低基线允许阈值电压电平被设置为非常接近MCP基线电压，从而允许在没有MCP基线噪声的过载或过度数字化的情况下获取尽可能多的信号。对Agilent调谐混合离子和重标记磷酸肽混合物的分析表明，14位ADC为高强度离子（如Agilent调谐混合离子和许多磷酸肽组分的2+和3+电荷状态）提供约1.5-2×信噪比（S/N）增加。然而，对于低强度离子，信号增强多达10倍，并且14位ADC能够实现可辨别的信号强度，否则使用8位数字转换器会丢失。此外，14位ADC所需的平均质谱数约为8位ADC的14倍，以产生具有类似S/N的质谱，显示出约10倍的测量吞吐量。新型14位ADC的高分辨率、低基线和快速特性使MS、IMS-MS和SLIM-IMS-MS光谱实现了高性能数字化，并提供了复杂混合物中分析物图谱的更好图像。
Signal digitization is a commonly overlooked part of ion mobility-mass spectrometry (IMS-MS) workflows, yet it greatly affects signal-to-noise ratio and MS resolution measurements. Here, we report on the integration of a 2 GS/s, 14-bit ADC with structures for lossless ion manipulations (SLIM-IMS-MS) and compare the performance to a commonly used 8-bit ADC. The 14-bit ADC provided a reduction in the digitized noise by a factor of ~6, owing largely to the use of smaller bit sizes. The low baseline allowed threshold voltage levels to be set very close to the MCP baseline voltage, allowing for as much signal to be acquired as possible without overloading or excessive digitization of MCP baseline noise. Analyses of Agilent tuning mixture ions and a mixture of heavy labeled phosphopeptides showed that the 14-bit ADC provided a ~1.5–2× signal-to-noise (S/N) increase for high intensity ions, such as the Agilent tuning mixture ions and the 2+ and 3+ charge states of many phosphopeptide constituents. However, signal enhancements were as much as 10-fold for low intensity ions, and the 14-bit ADC enabled discernible signal intensities otherwise lost using an 8-bit digitizer. Additionally, the 14-bit ADC required ~14-fold fewer mass spectra to be averaged to produce a mass spectrum with a similar S/N as the 8-bit ADC, demonstrating ~10× higher measurement throughput. The high resolution, low baseline, and fast speed of the new 14-bit ADC enables high performance digitization of MS, IMS-MS, and SLIM-IMS-MS spectra and provides a much better picture of analyte profiles in complex mixtures.
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DOI：
10.1016/j.jasms.2006.06.005
发表时间：
2006-09-01
影响因子：
3.2
作者：
Ibrahim, Yehia;Tang, Keqi;Smith, Richard D.
通讯作者：
Smith, Richard D.
影响因子：
1.3
作者：
Hondo, Toshinobu;Kawai, Yousuke;Toyoda, Michisato
通讯作者：
Toyoda, Michisato
DOI：
10.1039/c7an00031f
发表时间：
2017-03-27
期刊：
The Analyst
影响因子：
--
作者：
Ibrahim YM;Hamid AM;Deng L;Garimella SV;Webb IK;Baker ES;Smith RD
通讯作者：
Smith RD
DOI：
10.1021/jasms.1c00005
发表时间：
2021-04-07
影响因子：
3.2
作者：
Hollerbach AL;Conant CR;Nagy G;Monroe ME;Gupta K;Donor M;Giberson CM;Garimella SVB;Smith RD;Ibrahim YM
通讯作者：
Ibrahim YM
影响因子：
7.4
作者：
May, Jody C.;Knochenmuss, Richard;McLean, John A.
通讯作者：
McLean, John A.