Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites.
Assessment of Anopheles salivary antigens as individual exposure biomarkers to species-specific malaria vector bites.
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DOI：
10.1186/1475-2875-11-439
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发表时间：
2012-12-31
期刊：
影响因子：
3
通讯作者：
Almeras L
中科院分区：
文献类型：
--
作者：
Ali ZM;Bakli M;Fontaine A;Bakkali N;Vu Hai V;Audebert S;Boublik Y;Pagès F;Remoué F;Rogier C;Fraisier C;Almeras L
疟疾传播发生在受感染的按蚊吸血并同时将唾液注射到脊椎动物宿主体内的过程中。在撒哈拉以南非洲，大多数疟疾传播是由按蚊 s.s 和冈比亚按蚊 s.l 引起的。 （主要是冈比亚按蚊和阿拉伯按蚊）。多项研究表明，针对唾液抗原的免疫反应可用于评估个体是否接触过蚊虫叮咬。本研究的目的是评估分泌唾液蛋白作为接触 An 的特定生物标志物的用途。冈比亚和/或安。福内斯特虫咬伤。为此，使用了来自 An. 的唾液腺蛋白 6 (SG6) 和 5' 核苷酸酶 (5'nuc)。冈比亚 (gSG6 和 g-5′nuc) 和 An. funestus（fSG6 和 f-5'nuc）被选择并以重组形式生产。使用 ELISA 测试针对这些唾液蛋白的 IgG 反应的特异性，使用来自居住在三个塞内加尔村庄（NDiop，n = 50；Dielmo，n = 38；和 Diama，n = 46）的个体的血清进行测试，这些村庄曾暴露于不同密度和比例的按蚊物种。未接触过这些热带蚊子的个体被用作对照（马赛，n = 45）。 IgG 针对这两种按蚊物种的 SG6 重组蛋白以及针对按蚊的 g-5' 核苷酸酶作出反应。与未接触特定按蚊物种的对照组相比，塞内加尔个体中的冈比亚疟原虫显着更高。相反，观察到 An 水平之间存在关联。 funestus暴露和针对f-5'核苷酸酶蛋白的血清学免疫反应水平。这项研究揭示了按蚊唾液抗原蛋白，它可以被认为是一种有前途的抗原标记，可以在物种水平上区分疟疾媒介暴露。讨论了此类物种特异性抗原标记的流行病学意义。
Malaria transmission occurs during the blood feeding of infected anopheline mosquitoes concomitant with a saliva injection into the vertebrate host. In sub-Saharan Africa, most malaria transmission is due to Anopheles funestus s.s and to Anopheles gambiae s.l. (mainly Anopheles gambiae s.s. and Anopheles arabiensis). Several studies have demonstrated that the immune response against salivary antigens could be used to evaluate individual exposure to mosquito bites. The aim of this study was to assess the use of secreted salivary proteins as specific biomarkers of exposure to An. gambiae and/or An. funestus bites. For this purpose, salivary gland proteins 6 (SG6) and 5′nucleotidases (5′nuc) from An. gambiae (gSG6 and g-5′nuc) and An. funestus (fSG6 and f-5′nuc) were selected and produced in recombinant form. The specificity of the IgG response against these salivary proteins was tested using an ELISA with sera from individuals living in three Senegalese villages (NDiop, n = 50; Dielmo, n = 38; and Diama, n = 46) that had been exposed to distinct densities and proportions of the Anopheles species. Individuals who had not been exposed to these tropical mosquitoes were used as controls (Marseille, n = 45). The IgG responses against SG6 recombinant proteins from these two Anopheles species and against g-5′nucleotidase from An. gambiae, were significantly higher in Senegalese individuals compared with controls who were not exposed to specific Anopheles species. Conversely, an association was observed between the level of An. funestus exposure and the serological immune response levels against the f-5′nucleotidase protein. This study revealed an Anopheles salivary antigenic protein that could be considered to be a promising antigenic marker to distinguish malaria vector exposure at the species level. The epidemiological interest of such species-specific antigenic markers is discussed.
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影响因子：
15.9
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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作者：
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DOI：
10.4269/ajtmh.2010.09-0684
发表时间：
2010-07-01
影响因子：
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作者：
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