Almond Skin Extracts Abrogate HSV-1 Replication by Blocking Virus Binding to the Cell.
Almond Skin Extracts Abrogate HSV-1 Replication by Blocking Virus Binding to the Cell.
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DOI：
10.3390/v9070178
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发表时间：
2017-07-10
期刊：
影响因子：
--
通讯作者：
Sciortino MT
中科院分区：
文献类型：
--
作者：
Bisignano C;Mandalari G;Smeriglio A;Trombetta D;Pizzo MM;Pennisi R;Sciortino MT
本研究的目的是确定杏仁皮提取物对单纯疱疹病毒1型(HSV-1)复制的影响。在治疗后，HSV的抗药性毒株经常会出现。因此，抗单纯疱疹病毒新药的发现值得大力推进。在这里，我们测试了天然(NS)和漂白(BS)富含多酚的杏仁皮提取物对HSV-1的抑制作用。高效液相色谱分析表明，在NS和BS提取物中，对其抗氧化活性有贡献的主要化合物是槲皮素、表儿茶素和儿茶素。细胞存活率测定结果表明，NS和BS提取物对培养的Vero细胞没有毒性。此外，NS提取物比BS提取物对HSV-1的抑制作用更强，这一趋势与不同浓度的类黄酮一致。空斑形成试验、Western印迹和实时定量聚合酶链式反应(Real-Time-PCR)结果表明，NS提取物能够阻断HSV-1感染性颗粒的产生。病毒结合实验表明，NS提取物抑制了HSV-1在Vero细胞上的吸附。我们的结论是，杏仁皮提取物的天然产品是一种非同寻常的抗病毒药物来源，并提供了一种对抗HSV-1感染的新疗法。
The aim of the present research was to determine the effect of almond skin extracts on herpes simplex virus 1 (HSV-1) replication. Drug-resistant strains of HSV frequently develop following therapeutic treatment. Therefore, the discovery of novel anti-HSV drugs deserves great effort. Here, we tested both natural (NS) and blanched (BS) polyphenols-rich almond skin extracts against HSV-1. HPLC analysis showed that the prevalent compounds in NS and BS extracts contributing to their antioxidant activity were quercetin, epicatechin and catechin. Results of cell viability indicated that NS and BS extracts were not toxic to cultured Vero cells. Furthermore, NS extracts were more potent inhibitors of HSV-1 than BS extracts, and this trend was in agreement with different concentrations of flavonoids. The plaque forming assay, Western blot and real-time PCR were used to demonstrate that NS extracts were able to block the production of infectious HSV-1 particles. In addition, the viral binding assay demonstrated that NS extracts inhibited HSV-1 adsorption to Vero cells. Our conclusion is that natural products from almond skin extracts are an extraordinary source of antiviral agents and provide a novel treatment against HSV-1 infections.
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影响因子：
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通讯作者：
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