Optimizing the thermal read-out technique for MIP-based biomimetic sensors: towards nanomolar detection limits.
Optimizing the thermal read-out technique for MIP-based biomimetic sensors: towards nanomolar detection limits.
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DOI：
10.3390/s130709148
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发表时间：
2013-07-16
期刊：
影响因子：
--
通讯作者：
Wagner P
中科院分区：
文献类型：
--
作者：
Geerets B;Peeters M;van Grinsven B;Bers K;de Ceuninck W;Wagner P
在以前的工作中，提出了一种新的热传递方法（HTM）来检测带有分子印迹聚合物（MIP）型受体的小分子。在本研究中，我们将重点放在传感器性能的优化上，最终目标是通过降低噪声水平来降低检测极限。结果表明，噪声主要来源于电源，可通过改变PID参数进行控制。因此，通过在37°C的温度下分别调整P、I和D来评估各个参数的影响，给出了最佳配置的第一个指示。接下来，编制了温度剖面，并对一组参数研究了整个区域的传热阻力的标准差。与P10-I5-D0的原始参数相比，最佳配置P1-I6-D0将噪声水平降低了近三倍。利用优化后的设置对缓冲液中l -尼古丁的检测进行了研究，检测限由100 nM显著提高到35 nM。综上所述，优化PID参数从而提高检出限是htm方法首次应用于MIP受体分析研究的关键参数。
In previous work, the novel heat-transfer method (HTM) for the detection of small molecules with Molecularly Imprinted Polymers (MIP)-type receptors was presented. In this study we focus on optimization of this sensor performance, with as final aim to lower the detection limit by reducing the noise level. It was determined that the noise originates foremost from the power supply, which can be controlled by varying the PID parameters. Therefore, the effect of the individual parameters was evaluated by tuning P, I and D separately at a temperature of 37 °C, giving a first indication of the optimal configuration. Next, a temperature profile was programmed and the standard deviation of the heat-transfer resistance over the entire regime was studied for a set of parameters. The optimal configuration, P1-I6-D0, reduced the noise level with nearly a factor of three compared to the original parameters of P10-I5-D0. With the optimized settings, the detection of L-nicotine in buffer solutions was studied and the detection limit improved significantly from 100 nM to 35 nM. Summarizing, optimization of the PID parameters and thereby improving the detection limit is a key parameter for first applications of the HTM-method for MIP receptors in analytical research.
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