Porcine adenovirus as a delivery system for swine vaccines and immunotherapeutics.
Porcine adenovirus as a delivery system for swine vaccines and immunotherapeutics.
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DOI：
10.1016/j.tvjl.2003.09.007
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发表时间：
2005-01
期刊：
影响因子：
--
通讯作者：
Johnson MA
中科院分区：
文献类型：
--
作者：
Hammond JM;Johnson MA
猪腺病毒(PAdV)具有多种特性，使其成为猪体内理想的载体载体。它是一种低级别的病原体，几乎存在于世界各地的许多血清型中，其毒力和组织嗜性各不相同，这可能允许针对血清型的疫苗靶向。PAdV是只从猪身上分离出来的物种特异性病毒，减少了它在注射后传播到其他动物或人类的可能性。当设计成含有外源基因时，重组PAdV(RPAdV)可以在组织培养细胞中生长到高滴度，从而使其生产成本低廉。对PAdV基因组完整核苷酸序列的了解使得能够合理地定向插入外源基因，这些基因稳定地插入到基因组中，并可以在递送到目标宿主后高水平表达。重要的是，重组PAdV可以通过注射或口服途径在饲料或饮用水中给药。我们已经使用rPAdV将一系列抗原和免疫调节分子输送到商业上可以买到的猪身上，并发现它是一种非常有效的输送系统。值得注意的是，重组PAdV血清3型作为运送载体是非常有效的，即使在面对高水平的人工诱导的针对载体的血清特异性中和抗体的情况下也是如此。
Porcine adenovirus (PAdV) has many qualities which make it an ideal choice for use as a delivery vector in swine. It is a low grade pathogen, present almost world-wide in a number of serotypes varying in their virulence and tissue tropism, which may allow for serotype specific vaccine targeting. PAdV is species specific having only been isolated from swine, reducing the possibility of its spread to other animals or man following administration. When engineered to contain a foreign gene, recombinant PAdV (rPAdV) can be grown to high titres in tissue culture cells making it cheap to produce. Knowledge of the complete nucleotide sequence of the PAdV genome has enabled rationally directed insertions of foreign genes which remain stably inserted in the genome and can be expressed at high levels following delivery to the target host. Importantly, recombinant PAdV can be administered by injection or by the oral route in feed or drinking water. We have delivered a range of antigens and immunomodulatory molecules to commercially available pigs using rPAdV and found it to be a very effective delivery system. Significantly, recombinant PAdV serotype 3 is highly effective as a delivery vehicle even when administered in the face of high levels of artificially induced serotype specific neutralising antibody to the vector.
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影响因子：
3.3
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