Hepatic Gene Expression Changes in Rats Internally Exposed to Radioactive (56)MnO(2) Particles at Low Doses.
Hepatic Gene Expression Changes in Rats Internally Exposed to Radioactive (56)MnO(2) Particles at Low Doses.
复制标题
DOI：
10.3390/cimb43020055
复制
发表时间：
2021-07-22
影响因子：
3.1
通讯作者：
Fujimoto N
中科院分区：
文献类型：
--
作者：
Ruslanova B;Abishev Z;Chaizhunussova N;Shabdarbayeva D;Tokesheva S;Amantayeva G;Kairkhanova Y;Stepanenko V;Hoshi M;Fujimoto N
我们研究了放射性二氧化锰（56 MnO 2），主要的放射性同位素粉尘在土壤中发现原子弹爆炸后的内照射的生物效应。我们先前的血液化学研究表明，56 MnO 2可能对肝脏产生不良影响。在本研究中，我们通过测定肝脏基因表达的变化进一步研究了对肝脏的影响。将雄性Wistar大鼠暴露于56 MnO 2粒子（三组，全身剂量为41、90和100 m戈伊）、稳定MnO 2粒子或外部60 Co γ射线（2戈伊），并在暴露后第3天和第61天与未处理对照组一起进行检查。在肝脏中未观察到组织病理学变化。p53相关基因，细胞周期蛋白依赖性激酶抑制剂1A的mRNA表达在56 MnO 2和γ射线照射组中在照射后第3天和第61天增加。应激反应基因核因子κB的表达在应激后第3天也被56 MnO 2和γ射线所增加。然而，细胞因子基因（白细胞介素-6或趋化因子配体2）或纤维化相关TGF-β/Smad基因（Tgfb 1、Smad 3或Smad 4）的表达并未因暴露而改变。我们的数据表明，小于0.1戈伊的56 MnO 2粒子的内部暴露显著影响肝脏中的短期基因表达，其方式与2戈伊的外部γ辐射相似。这些变化可能是适应性反应，因为细胞因子或TGF-β/Smad基因表达没有发生变化。
We have studied the biological effects of the internal exposure to radioactive manganese-56 dioxide (56MnO2), the major radioisotope dust found in soil after atomic bomb explosions. Our previous study of blood chemistry indicated a possible adverse effect of 56MnO2 on the liver. In the present study, we further examined the effects on the liver by determining changes in hepatic gene expressions. Male Wistar rats were exposed to 56MnO2 particles (three groups with the whole-body doses of 41, 90, and 100 mGy), stable MnO2 particles, or external 60Co γ-rays (2 Gy), and were examined together with the non-treated control group on postexposure day 3 and day 61. No histopathological changes were observed in the liver. The mRNA expression of a p53-related gene, the cyclin-dependent kinase inhibitor 1A, increased in 56MnO2 as well as in γ-ray irradiated groups on postexposure day 3 and day 61. The expression of a stress-responsive gene, nuclear factor κB, was also increased by 56MnO2 and γ-rays on postexposure day 3. However, the expression of cytokine genes (interleukin-6 or chemokine ligand 2) or fibrosis-related TGF-β/Smad genes (Tgfb1, Smad3, or Smad4) was not altered by the exposure. Our data demonstrated that the internal exposure to 56MnO2 particles at less than 0.1 Gy significantly affected the short-term gene expressions in the liver in a similar manner with 2 Gy of external γ-irradiation. These changes may be adaptive responses because no changes occurred in cytokine or TGF-β/Smad gene expressions.
登录
查看更多内容
影响因子：
1.7
作者：
Stepanenko V;Kaprin A;Ivanov S;Shegay P;Zhumadilov K;Petukhov A;Kolyzhenkov T;Bogacheva V;Zharova E;Iaskova E;Chaizhunusova N;Shabdarbayeva D;Amantayeva G;Baurzhan A;Ruslanova B;Abishev Z;Apbassova M;Kairkhanova Y;Uzbekov D;Khismetova Z;Zhunussov Y;Fujimoto N;Sato H;Shichijo K;Nakashima M;Sakaguchi A;Toyoda S;Kawano N;Ohtaki M;Otani K;Endo S;Yamamoto M;Hoshi M
通讯作者：
Hoshi M
影响因子：
2
作者：
Imaeda M;Ishikawa H;Yoshida Y;Takahashi T;Ohkubo Y;Musha A;Komachi M;Nakazato Y;Nakano T
通讯作者：
Nakano T
影响因子：
--
作者：
Fujimoto, Nariaki;Suzuki, Tomoharu;Kitamura, Shigeyuki
通讯作者：
Kitamura, Shigeyuki
影响因子：
5.8
作者：
Ali, Ola Sayed M.;Amin, Nour El-Din;Abd El-Rahman, Omnia
通讯作者：
Abd El-Rahman, Omnia
影响因子：
2.6
作者：
Kabacik, Sylwia;Mackay, Alan;Badie, Christophe
通讯作者：
Badie, Christophe