Abundance of Class 1 Integron-Integrase and Sulfonamide Resistance Genes in River Water and Sediment Is Affected by Anthropogenic Pressure and Environmental Factors.
Abundance of Class 1 Integron-Integrase and Sulfonamide Resistance Genes in River Water and Sediment Is Affected by Anthropogenic Pressure and Environmental Factors.
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河水和沉积物中的1类整合子积聚酶和磺酰胺耐药基因受人为压力和环境因素的影响。
DOI：
10.1007/s00248-016-0843-4
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发表时间：
2016-11
影响因子：
3.6
通讯作者：
Lopacinska, Natalia
中科院分区：
文献类型：
--
作者：
Koczura, Ryszard;Mokracka, Joanna;Taraszewska, Agata;Lopacinska, Natalia
在这项研究中，我们确定了从污水处理厂废水排放上游和下游采样的河水和沉积物中分离出的可培养异养细菌中是否存在 1 类整合子整合酶基因。此外，我们使用 qPCR 定量了水和沉积物中的 intI1 和磺酰胺抗性基因（sul1 和 sul2）。水和沉积物样本的结果之间没有相关性，这表明含有整合子的细菌在这两个环境区室中的保留程度不同。处理后废水的排放显着增加了可培养细菌中intI1的频率和河水中的基因拷贝数，并增加了沉积物中sul1基因的数量。我们还观察到可培养异养生物中 1 类整合子整合酶基因的频率以及水中（但沉积物中）intI1 拷贝数的季节性差异。结果表明，水生栖息地中 1 类整合子的丰度取决于人为压力和环境因素。本文的在线版本 (doi:10.1007/s00248-016-0843-4) 包含补充材料，可供授权用户使用。
In this study, we determined the presence of class 1 integron-integrase gene in culturable heterotrophic bacteria isolated from river water and sediment sampled upstream and downstream of a wastewater treatment plant effluent discharge. Moreover, we quantified intI1 and sulfonamide resistance genes (sul1 and sul2) in the water and sediment using qPCR. There was no correlation between the results from water and sediment samples, which suggests integron-containing bacteria are differentially retained in these two environmental compartments. The discharge of treated wastewater significantly increased the frequency of intI1 among culturable bacteria and the gene copy number in river water, and increased the number of sul1 genes in the sediment. We also observed seasonal differences in the frequency of the class 1 integron-integrase gene among culturable heterotrophs as well as intI1 copy number in water, but not in sediment. The results suggest that the abundance of class 1 integrons in aquatic habitat depends on anthropogenic pressure and environmental factors. The online version of this article (doi:10.1007/s00248-016-0843-4) contains supplementary material, which is available to authorized users.
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影响因子：
13.6
作者：
Chen, Baowei;Liang, Ximei;Li, Xiangdong
通讯作者：
Li, Xiangdong
影响因子：
5.2
作者：
Barraud, O.;Baclet, M. C.;Ploy, M. C.
通讯作者：
Ploy, M. C.
DOI：
10.1038/ismej.2014.226
发表时间：
2015-06
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1038/ismej.2014.237
发表时间：
2015-06
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
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水产养殖环境中磺胺类、四环素类耐药细菌及耐药基因的存在
影响因子：
12.8
作者：
Gao, Panpan;Mao, Daqing;Xu, Lin
通讯作者：
Xu, Lin