Exercise and/or Genistein Do Not Revert 24-Week High-Fat, High-Sugar Diet-Induced Gut Microbiota Diversity Changes in Male C57BL/6J Adult Mice.
Exercise and/or Genistein Do Not Revert 24-Week High-Fat, High-Sugar Diet-Induced Gut Microbiota Diversity Changes in Male C57BL/6J Adult Mice.
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DOI：
10.3390/microorganisms10112221
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发表时间：
2022-11-10
期刊：
影响因子：
4.5
通讯作者：
Whisner CM
中科院分区：
文献类型：
--
作者：
Ortega-Santos CP;Al-Nakkash L;Whisner CM
肠道微生物群 (GM) 被认为是运动和饮食对健康有益的潜在调节因素。目前的文献主要关注运动和饮食的预防作用，探索这些治疗方法单独或组合是否可以通过肠道微生物群治疗肥胖，可能会受益。本研究旨在探讨金雀花素、运动及其协同作用对恢复饮食引起的肥胖和肠道微生物群变化的影响。总共 57 只雄性成年 C57BL/6 小鼠被随机分为 24 周的未纯化饮食（饲料）或高脂肪、高糖饮食（HFD；60% 脂肪总能量）。前 12 周后，接受 HFD 的动物被随机分为：HFD + 食物、HFD、HFD + 运动 (HFD + Exe)、HFD + 金雀异黄素 (HFD + Gen) 和 HFD + Exe + Gen。我们比较了 24 周后各组之间的体重变化。通过 Illumina MiSeq 对 16S rRNA 基因进行测序后，对 GM（α 多样性和 ß 多样性）进行了分析。相对于 HFD，HFD + Exe + Gen 显着（p < 0.05）降低了体重增加，仅 HFD + 食物将体重变化恢复到食物的体重变化。相对于 HFD + Gen 和 HFD + Exe 的饲料，包括 HFD 在内的所有饮食均降低了 GM 丰富度（观察到的扩增子序列变异），导致系统发育多样性显着低于 HFD。数据并不支持 HFD + Gen + Exe 给 GM 带来额外的好处。 HFD + Exe + Gen 在成年雄性小鼠中表现出更强的恢复饮食引起的肥胖的能力，但不如从 HFD 改为食物那么有效。对 HFD 引起的肥胖的生活方式治疗（包括运动和染料木黄酮）导致体重增加和 GM 丰富度减少，但从 HFD 改为食物最有可能将这些特征恢复到瘦对照。
The gut microbiota (GM) has been hypothesized to be a potential mediator in the health benefits of exercise and diet. The current literature is focused on the prevention effects of exercise and diet and could benefit from exploring whether these treatments alone or combined can treat obesity via the gut microbiome. This study aimed to explore the effects of genistein, exercise, and their synergistic effect to revert diet-induced obesity and gut microbiota changes. A total of 57 male adult C57BL/6 mice were randomized to 24 weeks of unpurified diet (chow) or a high-fat, high-sugar diet (HFD; 60% fat total energy). After the first 12 weeks, animals on the HFD were randomized into: HFD + chow, HFD, HFD + exercise (HFD + Exe), HFD + genistein (HFD + Gen), and HFD + Exe + Gen. We compared the body weight change between groups after 24 weeks. GM (α-diversity and ß-diversity) was profiled after sequencing the 16S rRNA gene by Illumina MiSeq. HFD + Exe + Gen significantly (p < 0.05) decreased weight gain relative to the HFD with only HFD + chow reverting the body weight change to that of chow. All diets including HFD reduced the GM richness (observed amplicon sequence variants) relative to chow with the HFD + Gen and HFD + Exe resulting in significantly lower phylogenetic diversity compared to the HFD. Data did not support an additive benefit to the GM for HFD + Gen + Exe. HFD + Exe + Gen showed a greater capacity to revert diet-induced obesity in adult male mice, but it was not as effective as switching from HFD to chow. Lifestyle treatment of HFD-induced obesity including exercise and genistein resulted in a reduction in weight gain and GM richness, but switching from HFD to chow had the greatest potential to revert these characteristics toward that of lean controls.
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DOI：
10.2147/dmso.s216312
发表时间：
2019-01-01
期刊：
DIABETES METABOLIC SYNDROME AND OBESITY-TARGETS AND THERAPY
影响因子：
--
作者：
Rockwood, Schuyler;Mason, Daniel;Al-Nakkash, Layla
通讯作者：
Al-Nakkash, Layla
大豆改善女性低拟合大鼠的心脏代谢健康和盲肠微生物群。
影响因子：
4.6
作者：
Cross TL;Zidon TM;Welly RJ;Park YM;Britton SL;Koch LG;Rottinghaus GE;de Godoy MRC;Padilla J;Swanson KS;Vieira-Potter VJ
通讯作者：
Vieira-Potter VJ
影响因子：
3.7
作者：
Gu S;Chen D;Zhang JN;Lv X;Wang K;Duan LP;Nie Y;Wu XL
通讯作者：
Wu XL
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
5.9
作者：
Ribeiro, Filipe M.;Ribeiro, Camila F. A.;Petriz, Bernardo A.
通讯作者：
Petriz, Bernardo A.