Sensitive and Specific DNA Detection Based on Nicking Endonuclease-Assisted Fluorescence Resonance Energy Transfer Amplification
Sensitive and Specific DNA Detection Based on Nicking Endonuclease-Assisted Fluorescence Resonance Energy Transfer Amplification
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基于切口核酸内切酶辅助荧光共振能量转移放大的灵敏且特异的 DNA 检测
DOI：
10.1021/jp2036263
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发表时间：
2011-08
影响因子：
3.7
通讯作者：
Xu, Chuanlai
中科院分区：
文献类型：
--
作者：
Xu, Liguang;Zhu, Yingyue;Ma, Wei;Kuang, Hua;Liu, Liqiang;Wang, Libing;Xu, Chuanlai
我们报告了一种通过切口核酸内切酶（NEase）辅助荧光共振能量转移（FRET）扩增检测靶DNA的方法。在本研究中，使用具有特异性识别核苷酸序列的靶DNA，其可以被NEase识别。NEase具有识别双链DNA中的特定核苷酸序列（接头DNA和靶DNA的杂交）并仅切割接头DNA的灵敏度和特异性。然后释放的靶DNA可以触发大摩尔过量的接头DNA的切割。靶DNA越多，切割的接头DNA越多，接头DNA诱导的FRET越少。发现该方法的检测范围为0.1至50 nM，检测限为0.077 nM。在此基础上，可以有效避免其他错配靶分子的干扰，甚至可以检测到NEase识别位点的单碱基突变。因此，我们建议NEASE辅助...
We report a method for the detection of target DNA through nicking endonuclease (NEase)-assisted fluorescence resonance energy transfer (FRET) amplification. In this study, target DNA with specific recognition nucleotide sequences was used, which could be recognized by NEase. NEase has the sensitivity and specificity to recognize specific nucleotide sequences in double-stranded DNA (the hybridization of linker DNA and target DNA) and cleave only the linker DNA. The released target DNA could then trigger the cleavage of a large molar excess of linker DNA. The more the target DNA and the more the cleaved linker DNA, the less the FRET was induced by the linker DNA. The detection range of this method was found to range from 0.1 to 50 nM, and the limit of detection was 0.077 nM. On the basis of this technology, the interference of other mismatched target molecules could be effectively avoided; even a single-base mutation at the NEase recognition site could be detected. We therefore propose that NEase-assisted ...
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影响因子：
3.5
作者：
E. Talavera;R. Bermejo;L. Crovetto;Á. Orte;J. M. Alvarez-Pez
通讯作者：
E. Talavera;R. Bermejo;L. Crovetto;Á. Orte;J. M. Alvarez-Pez
DOI：
10.1021/la703583u
发表时间：
2008-01
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
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作者：
Dejian Zhou;L. Ying;X. Hong;E. Hall;C. Abell;D. Klenerman
通讯作者：
Dejian Zhou;L. Ying;X. Hong;E. Hall;C. Abell;D. Klenerman
影响因子：
15
作者：
Peng, Hui;Zhang, Lijuan;Travas-Sejdic, Jadranka
通讯作者：
Travas-Sejdic, Jadranka
CdTe量子点阳极电化学发光及其能量转移检测儿茶酚衍生物
影响因子：
7.4
作者：
Liu, Xuan;Jiang, Hui;Ju, Huangxian
通讯作者：
Ju, Huangxian
影响因子：
4
作者：
K. Schallhorn;Katherine O. Freedman;Jessica M. Moore;Jun Lin;P. Ke
通讯作者：
K. Schallhorn;Katherine O. Freedman;Jessica M. Moore;Jun Lin;P. Ke