Interaction of transcriptional regulators with specific nucleosomes across the Saccharomyces genome.
Interaction of transcriptional regulators with specific nucleosomes across the Saccharomyces genome.
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DOI：
10.1016/j.molcel.2009.09.011
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发表时间：
2009-09-24
期刊：
影响因子：
16
通讯作者：
Pugh, B. Franklin
中科院分区：
文献类型：
--
作者：
Koerber, R. Thomas;Rhee, Ho Sung;Jiang, Cizhong;Pugh, B. Franklin
启动子周围的规范核小体结构为蛋白质调控基因表达提供了环境。与核小体相互作用的基因调控蛋白质是否对基因组中单个核小体位置具有选择性尚不清楚。在此，我们在酿酒酵母中研究了几种蛋白质 - 核小体相互作用，包括：1）结合组蛋白（Bdf1/SWR1和Srm1），2）结合特定DNA序列（Rap1和Reb1），以及3）在转录过程中可能与核小体发生碰撞（RNA聚合酶II）。我们发现Bdf1/SWR1复合物形成一种对活跃基因的+1和+2核小体具有选择性的双核小体复合物。Rap1选择性地结合到 -1核小体任一边界内DNA旋转暴露的第一和第二螺旋圈上其同源位点，而Reb1对 -1核小体靠近核小体缺失区域（NFR）的单个边界具有选择性。我们发现正在转录的RNA聚合酶使驻留核小体处于一种离域状态。这些发现表明启动子区域周围的核小体具有位置特异性功能，并且一些基因调控因子具有位置特异性的核小体相互作用。
A canonical nucleosome architecture around promoters establishes the context in which proteins regulate gene expression. Whether gene regulatory proteins that interact with nucleosomes are selective for individual nucleosome positions across the genome is not known. Here we examine in Saccharomyces several protein-nucleosome interactions, including those that 1) bind histones (Bdf1/SWR1 and Srm1), 2) bind specific DNA sequences (Rap1 and Reb1), and 3) potentially collide with nucleosomes during transcription (RNA polymerase II). We find that the Bdf1/SWR1 complex forms a di-nucleosome complex that is selective for the +1 and +2 nucleosomes of active genes. Rap1 selectively binds to its cognate site on the rotationally exposed first and second helical turn of DNA inside either border of the −1 nucleosome, whereas Reb1 is selective for a single NFR-proximal border of the −1 nucleosome. We find that a transcribing RNA polymerase creates a delocalized state of resident nucleosomes. These findings suggest that nucleosomes around promoter regions have position-specific functions, and that some gene regulators have position-specific nucleosomal interactions.
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影响因子：
16
作者：
Badis, Gwenael;Chan, Esther T.;van Bakel, Harm;Pena-Castillo, Lourdes;Tillo, Desiree;Tsui, Kyle;Carlson, Clayton D.;Gossett, Andrea J.;Hasinoff, Michael J.;Warren, Christopher L.;Gebbia, Marinella;Talukder, Shaheynoor;Yang, Ally;Mnaimneh, Sanie;Terterov, Dimitri;Coburn, David;Yeo, Ai Li;Yeo, Zhen Xuan;Clarke, Neil D.;Lieb, Jason D.;Ansari, Aseem Z.;Nislow, Corey;Hughes, Timothy R.
通讯作者：
Hughes, Timothy R.
影响因子：
5.3
作者：
Gelbart, ME;Rechsteiner, T;Tsukiyama, T
通讯作者：
Tsukiyama, T
影响因子：
30.8
作者：
Buck, Michael J.;Lieb, Jason D.
通讯作者：
Lieb, Jason D.
影响因子：
64.5
作者：
Hartley PD;Madhani HD
通讯作者：
Madhani HD
影响因子：
56.9
作者：
Dion, Michael F.;Kaplan, Tommy;Rando, Oliver J.
通讯作者：
Rando, Oliver J.