Noninvasive quantification of T2 and concentrations of ascorbate and glutathione in the human brain from the same double-edited spectra.
Noninvasive quantification of T2 and concentrations of ascorbate and glutathione in the human brain from the same double-edited spectra.
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DOI：
10.1002/nbm.1583
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发表时间：
2011-04
影响因子：
2.9
通讯作者：
Terpstra, Melissa
中科院分区：
文献类型：
--
作者：
Emir, Uzay E.;Deelchand, Dinesh;Henry, Pierre-Gilles;Terpstra, Melissa
横向弛豫时间（T2）和抗坏血酸（Asc）和谷胱甘肽（GSH）的浓度是通过双编辑的单一数据集测量的，这些数据集是在人脑中4 T的几个TEs下获得的。12名受试者每组扫描5次，使用102 - 152 ms之间的6个TEs计算T2。对所有六个TEs测量的光谱进行求和，以量化每次扫描中的浓度。优化了LCModel拟合，用于Asc和GSH双编辑光谱的定量分析。当拟合的基线被限制为平坦时，使用超过60次扫描测量的光谱总和，发现GSH的T2为67 ms(95%置信区间，50-83 ms)， Asc的T2≤115 ms。以10 μmol/g n -乙酰天冬氨酸为内参，并假设参比溶液和体内n -乙酰天冬氨酸和抗氧化剂T2弛豫的持续影响，60次扫描的Asc和GSH浓度分别为0.62±0.08和0.81±0.11 μmol/g [mean±standard deviation (SD), n = 60]。首次在人脑中测定GSH的T2值。这些数据与两种抗氧化剂的短T2一致。这些T2值对于长TE测量的Asc和GSH浓度的绝对定量是必不可少的，并且为解决关于T2的假设提供了关键步骤，从而实现了没有系统偏差可能性的浓度定量。
The transverse relaxation times (T2) and concentrations of Ascorbate (Asc) and glutathione (GSH) were measured from a single dataset of double-edited spectra that were acquired at several TEs at 4 T in the human brain. Six TEs between 102 and 152 ms were utilized to calculate T2 for the group of 12 subjects scanned five times each. Spectra measured at all six TEs were summed to quantify the concentration in each individual scan. LCModel fitting was optimized for the quantification of the Asc and GSH double-edited spectra. When the fitted baseline was constrained to be flat, T2 was found to be 67 ms (95% confidence interval, 50–83 ms) for GSH and ≤115 ms for Asc using the sum of spectra measured over 60 scans. The Asc and GSH concentrations quantified in each of the 60 scans were 0.62 ± 0.08 and 0.81 ± 0.11 μmol/g [mean ± standard deviation (SD), n = 60], respectively, using 10 μmol/g N-acetylaspartate as an internal reference and assuming a constant influence of N-acetylaspartate and antioxidant T2 relaxation in the reference solution and in vivo. The T2 value of GSH was measured for the first time in the human brain. The data are consistent with short T2 for both antioxidants. These T2 values are essential for the absolute quantification of Asc and GSH concentrations measured at long TE, and provide a critical step towards addressing assumptions about T2, and therefore towards the quantification of concentrations without the possibility of systematic bias.
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影响因子：
3.3
作者：
GRUETTER, R
通讯作者：
GRUETTER, R
影响因子：
3.3
作者：
Terpstra, M;Ugurbil, K;Gruetter, R
通讯作者：
Gruetter, R
影响因子：
3.3
作者：
KREIS, R;ERNST, T;ROSS, BD
通讯作者：
ROSS, BD
影响因子：
3.3
作者：
Henry, PG;Marjanska, M;Ugurbil, K
通讯作者：
Ugurbil, K
影响因子：
2.2
作者：
Pfeuffer, J;Tkác, I;Gruetter, R
通讯作者：
Gruetter, R