Effect of different factors on proliferation of antler cells, cultured in vitro.
Effect of different factors on proliferation of antler cells, cultured in vitro.
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DOI：
10.1371/journal.pone.0018053
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发表时间：
2011-03-28
期刊：
影响因子：
3.7
通讯作者：
Bubenik GA
中科院分区：
文献类型：
--
作者：
Kužmová E;Bartoš L;Kotrba R;Bubenik GA
鹿角作为骨骼生长和发育的潜在模型已成为人们越来越感兴趣的对象。为了阐明鹿茸生长是如何调节的过程，已经建立了体外培养。然而，迄今为止，还没有标准的方法来培养鹿茸细胞，体外结果往往与体内报道的结果相反。此外，许多在体外条件下通常未被考虑的因素可能在鹿茸细胞的发育中起重要作用。在这项研究中，我们调查了鹿茸生长阶段的影响，男性的个性，传代与原代培养物和胎牛血清浓度的影响，在体外增殖潜力的混合鹿茸细胞培养物，来自再生鹿角的红鹿男性（马鹿）。用~ 3 H胸腺嘧啶核苷掺入法测定鹿茸细胞的增殖能力。我们的研究结果表明，鹿茸生长阶段没有显着的影响，而男性的个性和所有其他检查的因素显着影响鹿茸细胞增殖。此外，我们的研究结果表明，原代培养可能更好地代表在体内的条件和过程中发生的再生鹿角。总之，在所有影响体外鹿茸细胞增殖的主要因素得到满意的研究之前，应极其谨慎地对待体外研究的结果，这些研究的重点是鹿茸生长的激素调节。
Antlers as a potential model for bone growth and development have become an object of rising interest. To elucidate processes explaining how antler growth is regulated, in vitro cultures have been established. However, until now, there has been no standard method to cultivate antler cells and in vitro results are often opposite to those reported in vivo. In addition, many factors which are often not taken into account under in vitro conditions may play an important role in the development of antler cells. In this study we investigated the effects of the antler growth stage, the male individuality, passaged versus primary cultures and the effect of foetal calf serum concentrations on proliferative potential of mixed antler cell cultures in vitro, derived from regenerating antlers of red deer males (Cervus elaphus). The proliferation potential of antler cells was measured by incorporation of 3H thymidine. Our results demonstrate that there is no significant effect of the antler growth stage, whereas male individuality and all other examined factors significantly affected antler cell proliferation. Furthermore, our results suggest that primary cultures may better represent in vivo conditions and processes occurring in regenerating antlers. In conclusion, before all main factors affecting antler cell proliferation in vitro will be satisfactorily investigated, results of in vitro studies focused on hormonal regulation of antler growth should be taken with extreme caution.
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影响因子：
3.6
作者：
Li, Chunyi;Mackintosh, Colin G.;Clark, Dawn E.
通讯作者：
Clark, Dawn E.
影响因子：
2.4
作者：
Colitti, M;Allen, SP;Price, JS
通讯作者：
Price, JS
DOI：
10.1007/s004290100204
发表时间：
2001-11-01
期刊：
ANATOMY AND EMBRYOLOGY
影响因子：
--
作者：
Li, CY;Suttie, JM
通讯作者：
Suttie, JM
影响因子：
2.7
作者：
Li, C;Wang, W;Suttie, JM
通讯作者：
Suttie, JM
影响因子：
3
作者：
Patel, Minal;Smith, Anthony J.;Cooper, Paul R.
通讯作者：
Cooper, Paul R.