Effects of Dietary Supplementation of Algae-Derived Polysaccharides on Morphology, Tight Junctions, Antioxidant Capacity and Immune Response of Duodenum in Broilers under Heat Stress.
Effects of Dietary Supplementation of Algae-Derived Polysaccharides on Morphology, Tight Junctions, Antioxidant Capacity and Immune Response of Duodenum in Broilers under Heat Stress.
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DOI：
10.3390/ani11082279
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发表时间：
2021-08-02
期刊：
影响因子：
--
通讯作者：
Yin FQ
中科院分区：
文献类型：
--
作者：
Liu WC;Zhu YR;Zhao ZH;Jiang P;Yin FQ
热应激已成为热带、亚热带地区家禽生产面临的巨大挑战。HS导致肉鸡肠道功能紊乱，严重影响其生产性能。前期研究表明，日粮中添加藻源多糖（ADP）可以促进肉鸡肠道屏障功能的恢复，但在HS条件下添加ADP对肉鸡肠道健康的影响尚不清楚。本研究表明，日粮中添加ADP可提高HS条件下肉鸡十二指肠紧密连接的表达，并通过调节Nrf 2和NF-κB信号通路减轻HS诱导的氧化应激和炎症反应。这些发现揭示了ADP作为HS缓解剂在维持肉鸡肠道健康方面的潜在应用。为探讨藻源多糖（ADP）对热应激引起的肉仔鸡十二指肠损伤的改善作用，试验选用144只雄性黄羽肉鸡（56日龄）随机分为三组：TN组HS组（热应激，肉鸡暴露于33.2 ± 1.5 ℃，10 h/d，8：00 a.m.-18：00 p.m.，其余时间温度与TN组一致）; HSA组（热应激肉鸡饲粮中添加1000 mg/kg ADP）。每个处理设6个重复，每个重复8只鸡。喂养试验持续了四周。结果表明，日粮中添加ADP能显著增加HS条件下肉鸡十二指肠绒毛高度（p = 0.077）和绒毛宽度（p = 0.062），降低细胞凋亡率（p = 0.081）。此外，日粮ADP还显著提高了HS条件下肉鸡十二指肠闭合蛋白和闭合小带1（ZO-1）mRNA和蛋白质的相对表达水平（p < 0.05）。此外，日粮ADP显著提高了HS条件下肉鸡十二指肠的总抗氧化能力（T-AOC）和谷胱甘肽-S转移酶（GST）活性，降低了十二指肠的丙二醛（MDA）含量（p < 0.05）。此外，日粮中添加ADP可显著上调热应激肉鸡十二指肠核因子红细胞2相关因子2（Nrf 2）、血红素氧合酶1（HO-1）、谷胱甘肽过氧化物酶1（GPx 1）和谷胱甘肽S转移酶θ 1（GSTT 1）mRNA的表达水平（p < 0.05）。此外，与HS组相比，添加ADP组肉鸡十二指肠抑制因子κ B α（IκBα）的相对mRNA表达量显著增加（p < 0.05），而肿瘤坏死因子-α（TNF-α）和白细胞介素-1 β（IL-1β）的相对mRNA表达量显著降低（p < 0.05）。综上所述，日粮中添加ADP对HS引起的肉鸡十二指肠紧密连接、抗氧化能力和免疫功能的损害具有改善作用。这些有益作用可能与调节Nrf 2和NF-κB信号通路有关。
Heat stress (HS) has become a great challenge for poultry production in tropical and subtropical regions. HS results in the intestinal dysfunction of broilers, which seriously affects their productivity. Our previous study suggested that dietary supplementation of algae-derived polysaccharides (ADP) could promote the intestinal barrier function in broilers, but the effect of dietary ADP supplementation on the intestinal health of broilers under HS remains unclear. The present study showed that dietary ADP supplementation improved the duodenal tight junction expression of broilers under HS, and found that dietary ADP mitigated HS-induced oxidative stress and inflammation response by regulating Nrf2 and NF-κB signaling pathways. These findings reveal the potential application of ADP as an HS-alleviating agent to maintain gut health in broilers. To evaluate the ameliorative effect of algae-derived polysaccharide (ADP) supplementation on duodenal injury caused by heat stress (HS) in broilers, a total of 144 male yellow-feathered broilers (56-day-old) were randomly allocated into three groups: The TN group (thermoneutral zone, broilers were raised at 23.6 ± 1.8 °C); HS group (heat stress, broilers were exposed to 33.2 ± 1.5 °C 10 h/day, 8:00 a.m.–18:00 p.m., the temperature in the remaining period was consistent with the TN group); HSA group (heat-stressed broilers were fed with ADP supplemented diet at 1000 mg/kg). There were six replications in each treatment, and eight broilers in each replication. The feeding trial lasted four weeks. The results showed that dietary ADP supplementation tended to increase the villus height (p = 0.077) and villus width (p = 0.062), and decrease the apoptosis rate (p = 0.081) in the duodenum of broilers under HS. Furthermore, dietary ADP increased the relative mRNA and protein (based on immunofluorescence) expression levels of occludin and zonula occludens-1 (ZO-1) in the duodenum of broilers under HS (p < 0.05). In addition, dietary ADP enhanced the total antioxidation capacity (T-AOC) and activity of glutathione-S transferase (GST), while reducing the malondialdehyde (MDA) concentration of the duodenum in broilers under HS (p < 0.05). Moreover, dietary ADP supplementation upregulated the duodenal nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), glutathione peroxidase 1 (GPx1) and glutathione S-transferase theta 1 (GSTT1) mRNA expression levels in heat-stressed broilers (p < 0.05). Furthermore, compared with the HS group, broilers fed with an ADP supplemented diet had a higher relative mRNA expression of inhibitor kappa B alpha (IκBα) (p < 0.05) and a lower relative mRNA expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the duodenum (p < 0.05). In summary, dietary ADP supplementation had an ameliorative effect on HS-induced impairment of tight junctions, antioxidant capacity and the immune response of the duodenum in broilers. These beneficial effects might be related to the modulation of Nrf2 and NF-κB signaling pathways.
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