The tendon injury response is influenced by decorin and biglycan.
The tendon injury response is influenced by decorin and biglycan.
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DOI：
10.1007/s10439-013-0915-2
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发表时间：
2014-03
影响因子：
3.8
通讯作者：
Soslowsky, Louis J.
中科院分区：
文献类型：
--
作者：
Dunkman, Andrew A.;Buckley, Mark R.;Mienaltowski, Michael J.;Adams, Sheila M.;Thomas, Stephen J.;Satchell, Lauren;Kumar, Akash;Pathmanathan, Lydia;Beason, David P.;Iozzo, Renato V.;Birk, David E.;Soslowsky, Louis J.
明确肌腱中的组成性调节分子对于理解肌腱修复过程以及指导新型治疗方法的开发至关重要。本研究的目的是明确肌腱损伤反应中的结构、表达和力学变化，并阐明两种I类富含亮氨酸小分子蛋白聚糖（SLRPs）的作用。我们利用双糖链蛋白聚糖基因敲除、核心蛋白聚糖基因敲除以及野生型小鼠，并建立了髌腱损伤模型。力学测试表明，损伤会导致功能变化，且六周后力学性能无法完全恢复。此外，SLRP缺乏会影响力学性能，核心蛋白聚糖基因敲除的肌腱在三到六周之间改善明显不足。对损伤反应和SLRPs作用的形态学分析表明，损伤会导致细胞密度和形状以及胶原排列和原纤维结构发生改变。通过实时定量聚合酶链反应（RT - qPCR）研究了SLRP基因表达，发现其表达变化与损伤肌腱相关。我们的研究结果表明，缺乏双糖链蛋白聚糖可能会损害初期愈合，而缺乏核心蛋白聚糖则会明显削弱后期愈合。这表明双糖链蛋白聚糖和核心蛋白聚糖在肌腱对损伤的反应中可能具有先后顺序的作用。
Defining the constituent regulatory molecules in tendon is critical to understanding the process of tendon repair and instructive to the development of novel treatment modalities. The purpose of this study is to define the structural, expressional, and mechanical changes in the tendon injury response, and elucidate the roles of two class I small leucine-rich proteoglycans (SLRPs). We utilized biglycan-null, decorin-null and wild type mice with an established patellar tendon injury model. Mechanical testing demonstrated functional changes associated with injury and the incomplete recapitulation of mechanical properties after six weeks. In addition, SLRP deficiency influenced the mechanical properties with a marked lack of improvement between three and six weeks in decorin-null tendons. Morphological analyses of the injury response and role of SLRPs demonstrated alterations in cell density and shape as well as collagen alignment and fibril structure resulting from injury. SLRP gene expression was studied using RT-qPCR with alterations in expression associated with the injured tendons. Our results show that in the absence of biglycan initial healing may be impaired while in the absence of decorin later healing is clearly diminished. This suggests that biglycan and decorin may have sequential roles in the tendon response to injury.
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DOI：
10.1115/1.4023234
发表时间：
2013-02-01
影响因子：
1.7
作者：
Connizzo, Brianne K.;Sarver, Joseph J.;Soslowsky, Louis J.
通讯作者：
Soslowsky, Louis J.
影响因子：
2.4
作者：
Ansorge, Heather L.;Adams, Sheila;Soslowsky, Louis J.
通讯作者：
Soslowsky, Louis J.
影响因子：
2.5
作者：
BIRK, DE;NURMINSKAYA, MV;ZYCBAND, EI
通讯作者：
ZYCBAND, EI
影响因子：
1.9
作者：
Berglund, Maria;Reno, Carol;Wiig, Monica
通讯作者：
Wiig, Monica
影响因子：
3
作者：
Young, MF;Bi, YM;Chen, XD
通讯作者：
Chen, XD