Nanozyme-Mediated Dual Immunoassay Integrated with Smartphone for Use in Simultaneous Detection of Pathogens.
Nanozyme-Mediated Dual Immunoassay Integrated with Smartphone for Use in Simultaneous Detection of Pathogens.
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DOI：
10.1021/acsami.7b12734
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发表时间：
2017-11-22
影响因子：
9.5
通讯作者：
Lin Y
中科院分区：
文献类型：
--
作者：
Cheng N;Song Y;Zeinhom MMA;Chang YC;Sheng L;Li H;Du D;Li L;Zhu MJ;Luo Y;Xu W;Lin Y
Nanozymes are an excellent class of optical reporters for the development of sensitive biosensors for widespread applications. In this study, mesoporous core–shell palladium@platinum (Pd@Pt) nanoparticles were synthesized and then applied as signal amplifier in a dual lateral flow immunoassay (LFIA) and integrated with a smartphone-based device for use in simultaneous detection of Salmonella Enteritidis and Escherichia coli O157:H7. After optimization, the limit of detections were calculated to be ~20 cfu/mL for S. Enteritidis and ~34 cfu/mL for E. coli O157:H7, respectively. The greatly improved sensitivity was contributed by the peroxidase-like catalytic activity of the Pd@Pt nanoparticles for signal enhancement and the parallel design of dual detection for eliminating the cross-interference. The estimated recoveries of the dual LFIA range from 91.44 to 117.00%, which indicated that the developed method is capable of detecting live bacteria in food samples. This approach provides an attractive platform for S. Enteritidis and E. coli O157:H7 detection using a smartphone-based device as the sole piece of equipment, indicating great promise for foodborne pathogen analysis or infield food safety tracking.
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DOI：
10.1038/nrmicro2949
发表时间：
2013-02
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3
作者：
Hossain, M. F.;Park, Jae Y.
通讯作者：
Park, Jae Y.
影响因子：
16.6
作者：
Ataee-Esfahani, Hamed;Imura, Masataka;Yamauchi, Yusuke
通讯作者：
Yamauchi, Yusuke
影响因子：
3.9
作者：
Hwang, Jeongin;Kwon, Donghoon;Jeon, Sangmin
通讯作者：
Jeon, Sangmin
影响因子：
9.5
作者：
Fenton, Erin M.;Mascarenas, Monica R.;Sibbett, Scott S.
通讯作者：
Sibbett, Scott S.