Identification of an intergenic region that is not essential for replication of goatpox virus

Identification of an intergenic region that is not essential for replication of goatpox virus
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鉴定对山羊痘病毒复制不是必需的基因间区域

DOI:
10.1007/s00705-010-0705-1
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发表时间:
2010-05
期刊:
Archives of Virology
影响因子:
--
通讯作者:
马文戈
马文戈
中科院分区:
其他
文献类型:
--
作者:
马文戈

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在11K晚启动子控制下插入增强型绿色荧光蛋白基因,在7.5K早/晚启动子控制下插入鸟嘌呤磷酸化基转移酶基因,并在重组基因组gp_24和gp_24.5位点之间的基因间区插入外源基因,构建了重组羊痘病毒。蛋白表达分析表明,仅重组病毒感染的LT细胞产生特异性荧光。比较生长试验证明了重组病毒在复制水平上的稳定性。这些结果表明,基因间区不是山羊痘病毒复制所必需的。
A recombinant goatpox virus was constructed in which an enhanced green fluorescent protein gene was inserted under the control of the 11K late promoter, a guanine phosphoribosyltransferase gene was inserted under the control of the 7.5K early/late promoter, and exogenous genes were inserted into an intergenic region between loci gp_24 and gp_24.5 of the recombinant genome. Analysis of protein expression showed that LT cells infected with only the recombinant virus produced specific fluorescence. A comparative growth assay demonstrated the stability of the recombinant virus at the replication level. These results suggest that the intergenic region is not essential for replication of goatpox virus.
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