Identification of an intergenic region that is not essential for replication of goatpox virus
Identification of an intergenic region that is not essential for replication of goatpox virus
复制标题
鉴定对山羊痘病毒复制不是必需的基因间区域
DOI:
10.1007/s00705-010-0705-1
复制
发表时间:
2010-05
期刊:
影响因子:
--
通讯作者:
马文戈
中科院分区:
文献类型:
--
作者:
马文戈
A recombinant goatpox virus was constructed in which an enhanced green fluorescent protein gene was inserted under the control of the 11K late promoter, a guanine phosphoribosyltransferase gene was inserted under the control of the 7.5K early/late promoter, and exogenous genes were inserted into an intergenic region between loci gp_24 and gp_24.5 of the recombinant genome. Analysis of protein expression showed that LT cells infected with only the recombinant virus produced specific fluorescence. A comparative growth assay demonstrated the stability of the recombinant virus at the replication level. These results suggest that the intergenic region is not essential for replication of goatpox virus.
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