Antimicrobial susceptibility of standard strains of nontuberculous mycobacteria by microplate Alamar Blue assay.
Antimicrobial susceptibility of standard strains of nontuberculous mycobacteria by microplate Alamar Blue assay.
复制标题
DOI：
10.1371/journal.pone.0084065
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wan K
中科院分区：
文献类型：
--
作者：
Li G;Lian LL;Wan L;Zhang J;Zhao X;Jiang Y;Zhao LL;Liu H;Wan K
在这项研究中，24个标准的非结核分枝杆菌（NTM）种菌株，包括12个缓慢生长的分枝杆菌菌株和12个快速生长的分枝杆菌菌株进行药物敏感性试验，使用微孔板Alcohol Blue法为基础的7H9肉汤。对24株NTM菌株最有效的抗菌药物为链霉素、阿米卡星、氟喹诺酮类和四环素类。龟分枝杆菌、结核分枝杆菌、博莱分枝杆菌和猿猴分枝杆菌对大多数抗菌剂具有耐药性。本研究的24株NTM标准菌株的药敏结果可供临床医生在对临床分离株进行药敏试验前或在条件不允许进行药敏试验时参考。临床和实验室标准化研究所推荐应用肉汤法，记录分枝杆菌标准菌株的药敏模式可以提高药敏试验方法的国际标准化。
In this study, 24 standard nontuberculous mycobacteria (NTM) species strains including 12 slowly growing mycobacteria strains and 12 rapidly growing mycobacteria strains were subjected to drug susceptibility testing using microplate Alamar Blue assay-based 7H9 broth. The most active antimicrobial agents against the 24 NTM strains were streptomycin, amikacin, the fluoroquinolones, and the tetracyclines. Mycobacterium chelonae, Mycobacterium abscessus, Mycobacterium bolletii, and Mycobacterium simiae are resistant to most antimicrobial agents. The susceptibility results of this study from 24 NTM standard strains can be referenced by clinicians before susceptibility testing for clinical isolates is performed or when conditions do not allow for susceptibility testing. The application of broth-based methods is recommended by the Clinical and Laboratory Standards Institute, and the documentation of the susceptibility patterns of standard strains of mycobacteria can improve the international standardization of susceptibility testing methods.
登录
查看更多内容
影响因子：
2.8
作者：
Petrini, Bjorn
通讯作者：
Petrini, Bjorn
影响因子：
4.9
作者：
WALLACE, RJ;BROWN, BA;ONYI, GO
通讯作者：
ONYI, GO
影响因子：
9.4
作者：
Richter, Elvira;Rusch-Gerdes, Sabine;Hillemann, Doris
通讯作者：
Hillemann, Doris
影响因子：
1.6
作者：
Gayathri, R.;Therese, Lily K.;Madhavan, H. N.
通讯作者：
Madhavan, H. N.
DOI：
10.1016/j.ijantimicag.2009.09.023
发表时间：
2010-02-01
影响因子：
10.8
作者：
van Ingen, Jakko;van der Laan, Tridia;van Soolingen, Dick
通讯作者：
van Soolingen, Dick