Distinct roles for classical nuclear import receptors in the growth of multinucleated muscle cells.
Distinct roles for classical nuclear import receptors in the growth of multinucleated muscle cells.
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DOI：
10.1016/j.ydbio.2011.06.032
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Pavlath GK
中科院分区：
文献类型：
--
作者：
Hall MN;Griffin CA;Simionescu A;Corbett AH;Pavlath GK
Proper muscle function is dependent on spatial and temporal control of gene expression in myofibers. Myofibers are multinucleated cells that are formed, repaired and maintained by the process of myogenesis in which progenitor myoblasts proliferate, differentiate and fuse. Gene expression is dependent upon proteins that require facilitated nuclear import, however little is known about the regulation of nucleocytoplasmic transport during the formation of myofibers. We analyzed the role of karyopherin alpha (KPNA), a key classical nuclear import receptor, during myogenesis. We established that five karyopherin alpha paralogs are expressed by primary mouse myoblasts in vitro and that their steady-state levels increase in multinucleated myotubes, suggesting a global increase in demand for classical nuclear import during myogenesis. We used siRNA-mediated knockdown to identify paralog-specific roles for KPNA1 and KPNA2 during myogenesis. KPNA1 knockdown increased myoblast proliferation, whereas KPNA2 knockdown decreased proliferation. In contrast, no proliferation defect was observed with KPNA4 knockdown. Only knockdown of KPNA2 decreased myotube growth. These results identify distinct pathways involved in myoblast proliferation and myotube growth that rely on specific nuclear import receptors suggesting that regulation of classical nuclear import pathways likely plays a critical role in controlling gene expression in skeletal muscle.
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影响因子：
2.6
作者：
Freitas N;Cunha C
通讯作者：
Cunha C
DOI：
10.1083/jcb.153.2.329
发表时间：
2001-04-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Horsley V;Friday BB;Matteson S;Kegley KM;Gephart J;Pavlath GK
通讯作者：
Pavlath GK
影响因子：
11.8
作者：
Griffin CA;Kafadar KA;Pavlath GK
通讯作者：
Pavlath GK
影响因子：
64.8
作者：
KALDERON, D;RICHARDSON, WD;SMITH, AE
通讯作者：
SMITH, AE
影响因子：
4.8
作者：
Datta, Kaustuv;Guan, Tinglu;Gerace, Larry
通讯作者：
Gerace, Larry