Culture of East Indian sandalwood tree somatic embryos in air-lift bioreactors for production of santalols, phenolics and arabinogalactan proteins
Culture of East Indian sandalwood tree somatic embryos in air-lift bioreactors for production of santalols, phenolics and arabinogalactan proteins
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DOI：
10.1093/aobpla/plt025
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发表时间：
2013-05-06
期刊：
影响因子：
2.9
通讯作者：
Dey S
中科院分区：
文献类型：
--
作者：
Misra BB;Dey S
印度檀香树因其珍贵的精油和心材而享誉全球。过度开发、疾病和栖息地丧失对寻找用于生物质生产的替代生物资源构成了重大挑战。在这里，我们报告的成功增长，在体外生长的体细胞胚胎在10升气升式生物反应器。 此外，我们的特点是阿拉伯半乳聚糖蛋白和小分子成分，如酚类和萜类物质分泌的悬浮体细胞胚到培养基中。在平行的生化表征，我们遵循整个发育进程的原胚性群众成熟子叶胚在一个单一的运行的生物反应器。东印度檀香树，檀香树，产量最昂贵的心材和珍贵的精油之一。毫不奇怪，由于全球需求未得到满足，非法贸易和采伐，过度采伐和流行性支原体穗病，这种濒危森林物种受到严重影响。体外微繁殖的努力已经导致了定义的体外阶段，如体细胞胚，适合在生物反应器中大规模生产。我们报告了体细胞胚胎生产在10升气升式生物反应器，并比较了生长和生化参数与2升气升式生物反应器。对于具有生物质（475.7 ± 18 g鲜重; P < 0.01）的10-L生物反应器，在28天内同时产生檀香醇（5.2 ± 0.15 mg L-1; P < 0.05）、酚类（31 ± 1.6 mg L-1）和阿拉伯半乳聚糖蛋白（AGP）（39 ± 3.1 mg L-1; P < 0.05）。此外，我们确定和定量的檀香醇和酚类化合物的高效薄层色谱法和反相高压液相色谱分析，分别通过。结果表明，10-L容量的气升式生物反应器能够支持体细胞胚培养，而细胞外培养基提供了生产工业原料，如檀香醇，酚类化合物和AGP的机会。这将被证明是有用的进一步优化和放大研究的植物产生的代谢产物。
The Indian Sandalwood tree is globally acclaimed for the precious essential oil and heartwood. Over-exploitation, diseases, and habitat loss have posed significant challenges to find an alternative bioresource for biomass production. Here, we report the successful growth of in vitro grown somatic embryos in 10 L air-lift bioreactors. Additionally, we characterized arabinogalactan proteins and small molecule constituents such as phenolics and terpenoids that are secreted by the suspended somatic embryos into the culture media. In parallel to the biochemical characterisation, we followed the entire developmental progression of proembryogenic masses into matured cotyledonary embryos during a single run of the bioreactor. The East Indian sandalwood tree, Santalum album, yields one of the costliest heartwoods and precious essential oil. Unsurprisingly, this endangered forest species is severely affected due to unmet global demands, illegal trade and harvesting, overharvesting and an epidemic mycoplasmal spike disease. In vitro micropropagation endeavours have resulted in defined in vitro stages such as somatic embryos that are amenable to mass production in bioreactors. We report on somatic embryo production in a 10-L air-lift-type bioreactor, and compare the growth and biochemical parameters with those of a 2-L air-lift-type bioreactor. For the 10-L bioreactor with biomass (475.7 ± 18 g fresh weight; P < 0.01), concomitantly santalols (5.2 ± 0.15 mg L−1; P < 0.05), phenolics (31 ± 1.6 mg L−1) and arabinogalactan proteins (AGPs) (39 ± 3.1 mg L−1; P < 0.05) are produced in 28 days. In addition, we identified and quantified several santalols and phenolics by means of high-performance thin-layer chromatography and reverse-phase high-pressure liquid chromatography analyses, respectively. Results indicate that 10-L-capacity air-lift bioreactors are capable of supporting somatic embryo cultures, while the extracellular medium provides opportunities for production of industrial raw materials such as santalols, phenolics and AGPs. This will prove useful for further optimization and scale-up studies of plant-produced metabolites.
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影响因子：
2.7
作者：
Godoy-Hernández, GC;Vázquez-Flota, FA;Loyola-Vargas, VM
通讯作者：
Loyola-Vargas, VM
影响因子：
2.9
作者：
Pomar, F;Merino, F;Barceló, AR
通讯作者：
Barceló, AR
影响因子：
7.4
作者：
Poon, Simon;Heath, Robyn Louise;Clarke, Adrienne Elizabeth
通讯作者：
Clarke, Adrienne Elizabeth
影响因子：
4.1
作者：
Howes, MJR;Simmonds, MSJ;Kite, GC
通讯作者：
Kite, GC
影响因子：
2.4
作者：
Misra, B. B.;Dey, S.
通讯作者：
Dey, S.