Construction of chromosomal recA mutants of Pseudomonas putida PpG2.

Construction of chromosomal recA mutants of Pseudomonas putida PpG2.
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恶臭假单胞菌PpG2染色体recA突变体的构建。

DOI:
10.1016/0378-1119(93)90476-j
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发表时间:
1993
期刊:
影响因子:
3.5
通讯作者:
Sokatch,JR
Sokatch,JR
中科院分区:
生物学3区
文献类型:
--
作者:
Luo,J;Burns,G;Sokatch,JR

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TherecAgene ofPseudomonas putidaPpG2 was cloned by complementation of therecAmutations ofEscherichia colistrains DH5α and HB1recAand a downstream partial open reading frame. Two mutants ofP. putidaPpG2, strains JS387 and JS388, were constructed by insertional inactivation ofrecAwith a tetracycline-resistance gene in both orientations. Both mutants acquired sensitivity to methyl methanesulfonate (MMS) and both failed to undergo homologous recombination. While therecAmutation ofP. putidaJS388 was complemented intransbyrecAofP. putida, the JS387 mutant was difficult to transform and transformants exhibited varying degrees of sensitivity to MMS. Therefore,P. putidaJS388 can be used as a carrier of recombinant plasmids, but JS387 is not a suitable host for this purpose.
DOI: 10.1073/pnas.86.6.1771
发表时间: 1989-03-01
影响因子: 11.1
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影响因子: 3.7
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