Reconstitution of a minimal motility system based on Spiroplasma swimming by two bacterial actins in a synthetic minimal bacterium.
Reconstitution of a minimal motility system based on Spiroplasma swimming by two bacterial actins in a synthetic minimal bacterium.
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DOI：
10.1126/sciadv.abo7490
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发表时间：
2022-12-02
期刊：
影响因子：
13.6
通讯作者：
中科院分区：
文献类型：
--
作者：
运动性是生命最重要的特征之一，但其进化起源仍然未知。在这项研究中，我们专注于螺原体，寄生虫或寄生菌。它们通过转换带状细胞骨架的螺旋度来游泳，这种细胞骨架由六种蛋白质组成，每种蛋白质都是从一种叫做MreB的核苷酶和细菌肌动蛋白进化而来的。我们在一个合成的，不动的最小的细菌，JCVI-syn 3B，其简化的基因组是计算机设计和化学合成的表达这些蛋白质。该合成菌具有螺旋体游动运动的特征。此外，螺原体MreB 4-MreB 5和MreB 1-MreB 5的组合产生螺旋形细胞形状和游泳。这些结果表明，游动起源于细菌肌动蛋白的分化和偶联，我们获得了合成细菌运动的最小系统。两种细菌肌动蛋白为合成的最小细菌提供细胞螺旋性和游泳能力。
Motility is one of the most important features of life, but its evolutionary origin remains unknown. In this study, we focused on Spiroplasma, commensal, or parasitic bacteria. They swim by switching the helicity of a ribbon-like cytoskeleton that comprises six proteins, each of which evolved from a nucleosidase and bacterial actin called MreB. We expressed these proteins in a synthetic, nonmotile minimal bacterium, JCVI-syn3B, whose reduced genome was computer-designed and chemically synthesized. The synthetic bacterium exhibited swimming motility with features characteristic of Spiroplasma swimming. Moreover, combinations of Spiroplasma MreB4-MreB5 and MreB1-MreB5 produced a helical cell shape and swimming. These results suggest that the swimming originated from the differentiation and coupling of bacterial actins, and we obtained a minimal system for motility of the synthetic bacterium. Two bacterial actins provide cell helicity and swimming capabilities to a synthetic minimal bacterium.
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影响因子：
28.3
作者：
Martin NR;Blackman E;Bratton BP;Chase KJ;Bartlett TM;Gitai Z
通讯作者：
Gitai Z
探索Spiroplasma Biology：机遇和挑战。
影响因子：
5.2
作者：
Harne S;Gayathri P;Béven L
通讯作者：
Béven L
DOI：
10.1016/j.bbrc.2005.03.168
发表时间：
2005-05-27
影响因子：
3.1
作者：
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通讯作者：
Uyeda, TQP
影响因子：
4.5
作者：
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通讯作者：
Blanchard A
影响因子：
5.2
作者：
Sasajima Y;Miyata M
通讯作者：
Miyata M