In Vivo Investigation of Polymer-Ceramic PCL/HA and PCL/β-TCP 3D Composite Scaffolds and Electrical Stimulation for Bone Regeneration.
In Vivo Investigation of Polymer-Ceramic PCL/HA and PCL/β-TCP 3D Composite Scaffolds and Electrical Stimulation for Bone Regeneration.
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DOI：
10.3390/polym14010065
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发表时间：
2021-12-25
期刊：
影响因子：
5
通讯作者：
Caetano GF
中科院分区：
文献类型：
--
作者：
Helaehil JV;Lourenço CB;Huang B;Helaehil LV;de Camargo IX;Chiarotto GB;Santamaria-Jr M;Bártolo P;Caetano GF
严重骨缺损是骨重建手术的主要临床挑战。聚己内酯（PCL）与羟基磷灰石（HA）和磷酸三钙（TCP）等生物陶瓷混合，形成具有更好生物识别性和生物活性的复合支架。电刺激（ES）旨在补偿受损的内源性电信号并刺激细胞增殖和分化。我们研究了复合支架（PCL与HA;和PCL与β-TCP）和ES的使用对Wistar大鼠关键骨缺损的影响，使用8个实验组：未处理组、ES组、PCL组、PCL/ES组、HA组、HA/ES组、TCP组和TCP/ES组。调查是基于组织形态计量学，免疫组织化学和基因表达分析。在第30天和第60天，HA/ES组的血管面积更大。在第30天，HA、HA/ES和TCP组的组织矿化更大，在第60天，TCP/ES组的组织矿化更大。BMP-2基因表达在第30天在HA、TCP和TCP/ES组中较高，在第60天在TCP/ES和PCL/ES组中较高。在第60天，TCP/ES组的Runx-2、Osterix和骨桥蛋白基因表达也较高。这些结果表明，打印有PCL和TCP的支架，当与电疗应用配对时，可改善骨再生。
Critical bone defects are a major clinical challenge in reconstructive bone surgery. Polycaprolactone (PCL) mixed with bioceramics, such as hydroxyapatite (HA) and tricalcium phosphate (TCP), create composite scaffolds with improved biological recognition and bioactivity. Electrical stimulation (ES) aims to compensate the compromised endogenous electrical signals and to stimulate cell proliferation and differentiation. We investigated the effects of composite scaffolds (PCL with HA; and PCL with β-TCP) and the use of ES on critical bone defects in Wistar rats using eight experimental groups: untreated, ES, PCL, PCL/ES, HA, HA/ES, TCP, and TCP/ES. The investigation was based on histomorphometry, immunohistochemistry, and gene expression analysis. The vascular area was greater in the HA/ES group on days 30 and 60. Tissue mineralization was greater in the HA, HA/ES, and TCP groups at day 30, and TCP/ES at day 60. Bmp-2 gene expression was higher in the HA, TCP, and TCP/ES groups at day 30, and in the TCP/ES and PCL/ES groups at day 60. Runx-2, Osterix, and Osteopontin gene expression were also higher in the TCP/ES group at day 60. These results suggest that scaffolds printed with PCL and TCP, when paired with electrical therapy application, improve bone regeneration.
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影响因子：
14
作者：
Ho-Shui-Ling A;Bolander J;Rustom LE;Johnson AW;Luyten FP;Picart C
通讯作者：
Picart C
DOI：
10.1016/j.dental.2015.09.008
发表时间：
2016-01
期刊：
Dental materials : official publication of the Academy of Dental Materials
影响因子：
--
作者：
Denry I;Kuhn LT
通讯作者：
Kuhn LT
DOI：
10.3390/ma11010129
发表时间：
2018-01-14
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Huang B;Caetano G;Vyas C;Blaker JJ;Diver C;Bártolo P
通讯作者：
Bártolo P
影响因子：
3.5
作者：
Chronopoulou, Laura;Cacciotti, Ilaria;Palocci, Cleofe
通讯作者：
Palocci, Cleofe
影响因子：
8.4
作者：
Chen, Shengyang;Jang, Tae-Sik;Song, Juha
通讯作者：
Song, Juha