MicroRNA Profiling of Red Blood Cells for Lung Cancer Diagnosis.
MicroRNA Profiling of Red Blood Cells for Lung Cancer Diagnosis.
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红细胞的microRNA分析用于肺癌诊断。
DOI：
10.3390/cancers15225312
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发表时间：
2023-11-07
期刊：
影响因子：
5.2
通讯作者：
Jiang, Feng
中科院分区：
文献类型：
--
作者：
Geng, Xinyan;Ma, Jie;Dhilipkannah, Pushpa;Jiang, Feng
尽管为确定肺癌的无细胞循环生物标志物做出了广泛的努力，但尚未实现临床应用。最近的证据表明，血细胞衍生的分子在肿瘤发生中起重要作用，并可能作为潜在的诊断生物标志物。在这项研究中，我们对三种主要血细胞类型进行microRNA（miRNA）分析：红细胞（RBC），外周血单核细胞和中性粒细胞，比较肺癌患者和健康对照组的样本。我们为每种细胞类型鉴定了不同的miRNA特征，并观察到患者和对照组之间的显著差异。我们首次表明RBC-miRNA有潜力作为肺癌的新生物标志物。通过构建基于细胞特异性miRNA的诊断面板，我们证明了整合来自多个细胞来源的miRNA比依赖于单个细胞类型提供了上级诊断准确性。背景：尽管广泛的努力，建立无细胞循环肺癌诊断的生物标志物，临床采用仍然难以捉摸。值得注意的是，新出现的证据表明红细胞（RBC）及其衍生物在肿瘤发生中发挥着关键作用，为使用血细胞衍生的微小RNA（miRNA）进行诊断进步开辟了潜在途径。研究方法：我们对26名肺癌患者和26名健康对照者的三种主要血细胞类型-红细胞、外周血单核细胞（PBMC）和嗜中性粒细胞进行了微阵列分析。使用液滴数字PCR在包括42个肺癌和39个对照病例的另外的群组内进行验证。结果：我们的研究在所有检查的血细胞类型中发现了与肺癌相关的不同miRNA谱。有趣的是，RBC-miRNAs成为肺癌的潜在新型生物标志物，这一观察结果尚未被记录。重要的是，整合来自不同血细胞类型的miRNA产生了上级单个细胞类型miRNA的肺癌诊断准确性。随后，我们制定了三个诊断面板，熟练地识别非小细胞肺癌，腺癌和鳞状细胞癌，在不同的疾病阶段保持一致性。结论：RBC衍生的分子引入了新的癌症生物标志物，并且利用不同血细胞类型的miRNA谱揭示了肺癌早期检测和组织学分类的有希望的前沿。
Despite extensive efforts to identify cell-free circulating biomarkers for lung cancer, none have yet achieved clinical adoption. Recent evidence suggests that blood cell-derived molecules play significant roles in tumorigenesis and may serve as potential diagnostic biomarkers. In this study, we perform microRNA (miRNA) profiling on three primary blood cell types: red blood cells (RBCs), peripheral blood mononuclear cells, and neutrophils, comparing samples from lung cancer patients to healthy controls. We identify distinct miRNA signatures for each cell type and observe significant differences between patient and control cohorts. We show for the first time that RBC-miRNAs have potential as novel biomarkers for lung cancer. By constructing diagnostic panels based on cell-specific miRNAs, we demonstrate that integrating miRNAs from multiple cell sources offers superior diagnostic accuracy than relying on a single cell type. Background: Despite extensive endeavors to establish cell-free circulating biomarkers for lung cancer diagnosis, clinical adoption remains elusive. Noteworthy, emergent evidence suggests the pivotal roles of red blood cells (RBCs) and their derivatives in tumorigenesis, illuminating potential avenues for diagnostic advancements using blood cell-derived microRNAs (miRNAs). Methods: We executed microarray analyses on three principal blood cell types—RBCs, peripheral blood mononuclear cells (PBMCs), and neutrophils—encompassing 26 lung cancer patients and 26 healthy controls. Validation was performed using droplet digital PCR within an additional cohort comprising 42 lung cancer and 39 control cases. Results: Our investigation unearthed distinct miRNA profiles associated with lung cancer across all examined blood cell types. Intriguingly, RBC-miRNAs emerged as potential novel biomarkers for lung cancer, an observation yet to be documented. Importantly, integrating miRNAs from disparate blood cell types yielded a superior diagnostic accuracy for lung cancer over individual cell-type miRNAs. Subsequently, we formulated three diagnostic panels, adeptly discerning non-small cell lung cancer, adenocarcinoma, and squamous cell carcinoma, maintaining consistency across various disease stages. Conclusion: RBC-derived molecules introduce novel cancer biomarkers, and exploiting miRNA profiles across varied blood cell types unveils a promising frontier for lung cancer’s early detection and histological classification.
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影响因子：
6.6
作者：
Helwa, Reham;Heller, Anette;Bauer, Andrea S.
通讯作者：
Bauer, Andrea S.
DOI：
10.4137/bic.s37333
发表时间：
2016
期刊：
Biomarkers in cancer
影响因子：
--
作者：
Ma J;Li N;Lin Y;Gupta C;Jiang F
通讯作者：
Jiang F
影响因子：
2.3
作者：
Hernandez-Hernandez, Angel;Rodriguez, Marina C.;Sanchez-Yague, Jesus
通讯作者：
Sanchez-Yague, Jesus
影响因子：
3.8
作者：
Ma, Jie;Li, Ning;Jiang, Feng
通讯作者：
Jiang, Feng
影响因子：
3.7
作者：
Hunter, Melissa Piper;Ismail, Noura;Zhang, Xiaoli;Aguda, Baltazar D.;Lee, Eun Joo;Yu, Lianbo;Xiao, Tao;Schafer, Jeffrey;Lee, Mei-Ling Ting;Schmittgen, Thomas D.;Nana-Sinkam, S. Patrick;Jarjoura, David;Marsh, Clay B.
通讯作者：
Marsh, Clay B.