Perspective on the application of genome sequencing for monkeypox virus surveillance.
Perspective on the application of genome sequencing for monkeypox virus surveillance.
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DOI:
10.1016/j.virs.2023.03.006
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发表时间:
2023-04
影响因子:
5.5
通讯作者:
Tan, Wenjie
中科院分区:
文献类型:
--
作者:
Chen, Yuda;Wu, Changcheng;Ruhan, A.;Zhao, Li;Zhang, Zhongxian;Tan, Wenjie
Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity. The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained. Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated. Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.
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DOI:
10.3390/v12111257
发表时间:
2020-11-05
期刊:
Viruses
影响因子:
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作者:
Alakunle E;Moens U;Nchinda G;Okeke MI
通讯作者:
Okeke MI
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影响因子:
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通讯作者:
Buller, RML
DOI:
10.1126/science.add4153
发表时间:
2022-11-04
期刊:
Science (New York, N.Y.)
影响因子:
--
作者:
通讯作者:
--
DOI:
10.1093/bioinformatics/bty560
发表时间:
2018-09-01
期刊:
Bioinformatics (Oxford, England)
影响因子:
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作者:
Chen S;Zhou Y;Chen Y;Gu J
通讯作者:
Gu J