Absolute quantification of a plasma tRNA-derived fragment for the diagnosis and prognosis of gastric cancer.
Absolute quantification of a plasma tRNA-derived fragment for the diagnosis and prognosis of gastric cancer.
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DOI：
10.3389/fonc.2023.1106997
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发表时间：
2023
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
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作者：
从健康的胃黏膜到胃癌的转变是一个多步骤的过程。早期筛查可显著提高胃癌患者的生存率。迫切需要可靠的液体活检来预测胃癌，由于trna衍生片段（tRFs）在各种体液中丰富，tRFs可能是胃癌的新生物标志物。收集不同胃粘膜病变患者及健康人血浆样本438份。设计了特异的逆转录引物、正向引物、反向引物和TaqMan探针。构建标准曲线，设计绝对定量方法检测不同胃粘膜病变个体血浆样品中tRF-33-P4R8YP9LON4VDP含量。构建受试者工作特征曲线，评价tRF-33-P4R8YP9LON4VDP对不同胃粘膜个体的诊断价值。建立Kaplan-Meier曲线，计算tRF-33-P4R8YP9LON4VDP对晚期胃癌患者的预后价值。最后进行多因素Cox回归分析，评估tRF-33-P4R8YP9LON4VDP对晚期胃癌患者的独立预后价值。成功建立了血浆tRF-33-P4R8YP9LON4VDP检测方法。血浆tRF-33-P4R8YP9LON4VDP水平反映了从健康个体到胃炎患者到早期和晚期胃癌患者的梯度变化。不同胃粘膜个体之间存在显著差异，tRF-33-P4R8YP9LON4VDP水平降低与预后不良显著相关。发现tRF-33-P4R8YP9LON4VDP是不利生存结果的独立预测因子。在本研究中，我们开发了一种血浆tRF-33-P4R8YP9LON4VDP定量检测方法，该方法具有超敏性、便利性和特异性。发现tRF-33-P4R8YP9LON4VDP的检测是监测不同胃黏膜和预测患者预后的重要手段。
The transition from a healthy gastric mucosa to gastric cancer is a multi-step process. Early screening can significantly improve the survival rate of gastric cancer patients. A reliable liquid biopsy for gastric cancer prediction is urgently needed and since tRNA-derived fragments (tRFs) are abundant in various body fluids, tRFs are possible new biomarkers for gastric cancer. A total of 438 plasma samples from patients with different gastric mucosal lesions as well as healthy individuals were collected. A specific reverse transcription primer, a forward primer, a reverse primer, and a TaqMan probe were designed. A standard curve was constructed and an absolute quantitation method was devised for detection of tRF-33-P4R8YP9LON4VDP in plasma samples of individuals with differing gastric mucosa lesions. Receiver operating characteristic curves were constructed to evaluate the diagnostic values of tRF-33-P4R8YP9LON4VDP for individual with differing gastric mucosa. A Kaplan–Meier curve was established to calculate the prognostic value of tRF-33-P4R8YP9LON4VDP for advanced gastric cancer patients. Finally, a multivariate Cox regression analysis was performed to assess the independent prognostic value of tRF-33-P4R8YP9LON4VDP for advanced gastric cancer patients. A detection method for plasma tRF-33-P4R8YP9LON4VDP was successfully established. Levels of plasma tRF-33-P4R8YP9LON4VDP were shown to reflect a gradient change from healthy individuals to gastritis patients to early and advanced gastric cancer patients. Significant differences were found among individuals with differing gastric mucosa, with reduced levels of tRF-33-P4R8YP9LON4VDP significantly related to a poor prognosis. tRF-33-P4R8YP9LON4VDP was found to be an independent predictor of an unfavorable survival outcome. In this study, we developed a quantitative detection method for plasma tRF-33-P4R8YP9LON4VDP that exhibited hypersensitivity, convenience, and specificity. Detection of tRF-33-P4R8YP9LON4VDP was found to be a valuable means by which to monitor different gastric mucosa and to predict patient prognosis.
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影响因子：
7.3
作者：
Yang P;Zhang X;Chen S;Tao Y;Ning M;Zhu Y;Liang J;Kong W;Shi B;Li Z;Shen H;Wang Y
通讯作者：
Wang Y
应激诱导的 tRNA 衍生 RNA：原始真核生物兰氏贾第鞭毛虫中的一类新型小 RNA。
影响因子：
14.9
作者：
Li Y;Luo J;Zhou H;Liao JY;Ma LM;Chen YQ;Qu LH
通讯作者：
Qu LH
DOI：
10.1007/s00109-022-02189-0
发表时间：
2022-05
期刊：
Journal of molecular medicine (Berlin, Germany)
影响因子：
--
作者：
通讯作者：
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血清 hsa_tsr016141 作为一种 tRNA 衍生片段是胃癌的新型生物标志物
影响因子：
4.7
作者：
Gu X;Ma S;Liang B;Ju S
通讯作者：
Ju S
tRNA 衍生片段 tRF-17-79MP9PP 通过 THBS1/TGF-b1/Smad3 轴减弱乳腺癌中的细胞侵袭和迁移
影响因子：
4.7
作者：
Mo D;He F;Zheng J;Chen H;Tang L;Yan F
通讯作者：
Yan F