Serum hsa_tsr016141 as a Kind of tRNA-Derived Fragments Is a Novel Biomarker in Gastric Cancer.
Serum hsa_tsr016141 as a Kind of tRNA-Derived Fragments Is a Novel Biomarker in Gastric Cancer.
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血清 hsa_tsr016141 作为一种 tRNA 衍生片段是胃癌的新型生物标志物
DOI：
10.3389/fonc.2021.679366
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发表时间：
2021
影响因子：
4.7
通讯作者：
Ju S
中科院分区：
文献类型：
--
作者：
Gu X;Ma S;Liang B;Ju S
背景胃癌是全球最常见的恶性肿瘤之一，也是癌症相关死亡的第三大原因。目前，胃癌诊断标志物的敏感性和特异性较低，迫切需要寻找更高敏感性和特异性的新的生物标志物。TRNA来源的小RNA是一种来源于tRNA的非编码小RNA。它在癌细胞和体液中含量丰富。我们的目标是寻找在胃癌中差异表达的tRNA衍生的小RNA，以探索它们作为胃癌生物标志物的潜力。方法采用实时定量聚合酶链式反应检测hsA_tsr016141基因的表达水平。用琼脂糖凝胶电泳法、Sanger测序、放线菌素D试验、核质RNA分离试验等方法对hsa_tsr016141的分子特征进行了验证。通过接收器的工作特性分析了hsa_tsr016141的诊断效率。结果hsA_tsr016141在胃癌组织和血清中的表达水平显著升高。胃癌患者、胃炎患者和健康体检者的血清表达水平呈梯度变化，且与淋巴结转移程度和肿瘤分级呈正相关。ROC分析显示，hsa_tsr016141的血清表达水平可以明显区分胃癌患者与健康献血员或胃炎患者。此外，胃癌患者术后hsa_tsr016141的表达水平明显下降(P<；0.0001)。结论血清hsa_tsr016141具有良好的稳定性和特异性，可用于胃癌患者的动态监测，提示血清hsa_tsr016141可作为胃癌诊断和术后监测的一种新的生物标志物。
Background Gastric cancer (GC) is one of the most common malignant tumors globally and the third leading cause of cancer-related death. Currently, the sensitivity and specificity of diagnostic markers for GC are low, so it is urgent to find new biomarkers with higher sensitivity and specificity. tRNA-derived small RNAs are a kind of small non-coding RNAs derived from tRNAs. It is abundant in cancer cells and body fluids. Our goal is to find the differentially expressed tRNA-derived small RNAs in GC to explore their potential as a GC biomarker. Methods Quantitative real-time PCR was used to detect the expression level of hsa_tsr016141. The molecular characteristics of hsa_tsr016141 were verified by agarose gel electrophoresis, Sanger sequencing, Actinomycin D Assay, and Nuclear and Cytoplasmic RNA Separation Assay. The diagnostic efficiency of hsa_tsr016141 was analyzed through receiver operating characteristic. Results The expression level of hsa_tsr016141 in GC tissues and serum was significantly increased. The serum expression level showed a gradient change between GC patients, gastritis patients, and healthy donors and was positively correlated with the degree of lymph node metastasis and tumor grade. ROC analysis showed that the serum expression level of hsa_tsr016141 could significantly distinguish GC patients from healthy donors or gastritis patients. Besides, the expression level of hsa_tsr016141 in GC patients decreased significantly after the operation (P<0.0001). Conclusions Serum hsa_tsr016141 has good stability and specificity and can be used for dynamic monitoring of GC patients, suggesting that serum hsa_tsr016141 can be a novel biomarker for GC diagnosis and postoperative monitoring.
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影响因子：
64.5
作者：
Goodarzi H;Liu X;Nguyen HC;Zhang S;Fish L;Tavazoie SF
通讯作者：
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影响因子：
2
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作者：
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2.2
作者：
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通讯作者：
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作者：
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通讯作者：
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