Distinct temporal recruitment of Plasmodium alveolins to the subpellicular network.
Distinct temporal recruitment of Plasmodium alveolins to the subpellicular network.
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DOI：
10.1007/s00436-014-4093-4
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发表时间：
2014-11
影响因子：
2
通讯作者：
Dessens JT
中科院分区：
文献类型：
--
作者：
Tremp AZ;Al-Khattaf FS;Dessens JT
疟原虫的子孢子阶段（裂殖子、动合子和子孢子）具有称为表膜的独特皮质结构，其由称为内膜复合物（IMC）的双膜层限定。IMC由称为膜下网络（SPN）的中间丝的细胞骨架支持。疟原虫IMC 1蛋白或肺泡蛋白组成结构相关蛋白的保守家族，其包含SPN的构建块。在这里，使用绿色荧光蛋白（GFP）标记在伯氏疟原虫，我们表明，肺泡蛋白PbIMC 1c和PbIMC 1e的表达在所有三个子期。我们的数据显示，PbIMC 1e组装成SPN与薄膜发展的同时，而PbIMC 1c组装后，薄膜形成。在有性阶段，这些过程伴随着来自母本和父本等位基因的不同基因表达：PbIMC 1e唯一地从母本等位基因表达，而PbIMC 1c在配子母细胞中从母本等位基因表达，但在动合子发育期间从两个亲本等位基因表达。这些研究结果建立疟原虫皮层细胞骨架的生物发生是一个复杂的和动态的过程，涉及不同的亲本基因表达和按时间顺序招募其蛋白质成分。虽然使用双交换同源重组很容易实现pbimc1c和pbimc1e基因与GFP标记版本的等位基因置换，但通过这种策略破坏这些基因的尝试仅导致选择性标记和GFP报告基因整合到非特异性基因组位置。经常无法破坏这些基因提供了第一个遗传证据，肺泡蛋白是必要的无性血液阶段的疟原虫发展。
The zoite stages of malaria parasites (merozoite, ookinete and sporozoite) possess a distinctive cortical structure termed the pellicle, which is defined by a double membrane layer named the inner membrane complex (IMC). The IMC is supported by a cytoskeleton of intermediate filaments, termed the subpellicular network (SPN). Plasmodium IMC1 proteins, or alveolins, make up a conserved family of structurally related proteins that comprise building blocks of the SPN. Here, using green fluorescent protein (GFP) tagging in P. berghei, we show that the alveolins PbIMC1c and PbIMC1e are expressed in all three zoite stages. Our data reveal that PbIMC1e is assembled into the SPN concurrent with pellicle development, while PbIMC1c is assembled after pellicle formation. In the sexual stages, these processes are accompanied by different gene expressions from maternal and paternal alleles: PbIMC1e is expressed uniquely from the maternal allele, while PbIMC1c is expressed from the maternal allele in gametocytes, but from both parental alleles during ookinete development. These findings establish biogenesis of the cortical cytoskeleton in Plasmodium to be a complex and dynamic process, involving distinct parental gene expression and chronological recruitment of its protein constituents. While allelic replacement of the pbimc1c and pbimc1e genes with GFP-tagged versions was readily achieved using double crossover homologous recombination, attempts to disrupt these genes by this strategy only resulted in the integration of the selectable marker and GFP reporter into non-specific genomic locations. The recurrent inability to disrupt these genes provides the first genetic evidence that alveolins are necessary for asexual blood-stage parasite development in Plasmodium.
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影响因子：
1.5
作者：
Saeed S;Tremp AZ;Dessens JT
通讯作者：
Dessens JT
影响因子：
7
作者：
Lindner, Scott E.;Swearingen, Kristian E.;Kappe, Stefan H. I.
通讯作者：
Kappe, Stefan H. I.
影响因子：
64.8
作者：
Lasonder, E;Ishihama, Y;Mann, M
通讯作者：
Mann, M
影响因子：
2
作者：
MEIS, JFGM;POOL, G;MEUWISSEN, JHET
通讯作者：
MEUWISSEN, JHET
影响因子：
1.5
作者：
Saeed, Sadia;Carter, Victoria;Dessens, Johannes T.
通讯作者：
Dessens, Johannes T.