Dendritic morphology and inhibitory regulation distinguish dentate semilunar granule cells from granule cells through distinct stages of postnatal development.
Dendritic morphology and inhibitory regulation distinguish dentate semilunar granule cells from granule cells through distinct stages of postnatal development.
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DOI：
10.1007/s00429-020-02162-y
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发表时间：
2020-12
影响因子：
3.1
通讯作者：
Santhakumar V
中科院分区：
文献类型：
--
作者：
Gupta A;Proddutur A;Chang YJ;Raturi V;Guevarra J;Shah Y;Elgammal FS;Santhakumar V
半月颗粒细胞（SGCs）被认为是幼鼠海马齿状突起神经元的一种形态功能独特的类型，参与反馈抑制和记忆加工。然而，能够通过出生后发育可靠地将颗粒细胞（GCs）与SGCs分类的结构和生理特征仍未得到解决。以出生后11-13天、28-42天和出生后120天（对应于人类婴儿期、青春期和成人期）为研究对象，我们检测了SGCs和GCs的体突形态和抑制调控，以确定细胞类型特异性特征。无监督聚类分析证实，无论动物年龄如何，形态学特征都能可靠地区分SGCs和GCs。从婴儿期到成年期，SGCs比GCs保持更高的自发抑制性突触后电流（sIPSC）频率。虽然sIPSC频率在青春期特别增强，但sIPSC振幅和累积电荷转移从婴儿期到成年期下降，并且在gc和SGCs之间没有差异。两种细胞类型的突触外GABA电流在青春期均达到峰值，且上颌神经细胞的GABA电流在青春期显著高于上颌神经细胞。虽然在婴儿期和青春期GC的输入阻抗高于SGCs，但随着发育年龄的增长，GCs的输入阻抗下降，而SGCs的输入阻抗逐渐增加。因此，成人GCs的输入电阻明显低于SGCs。这些数据描述了结构特征，可以通过发育可靠地区分gc和SGCs。结果揭示了GCs和SGCs在被动膜特性和稳态抑制方面的发育差异，这可能混淆了它们在细胞类型分类中的应用。
Semilunar granule cells (SGCs) have been proposed as a morpho-functionally distinct class of hippocampal dentate projection neurons contributing to feedback inhibition and memory processing in juvenile rats. However, the structural and physiological features that can reliably classify granule cells (GCs) from SGCs through postnatal development remain unresolved. Focusing on postnatal days 11-13, 28-42, and >120, corresponding with human infancy, adolescence, and adulthood, we examined the somatodendritic morphology and inhibitory regulation in SGCs and GCs to determine the cell-type specific features. Unsupervised cluster analysis confirmed that morphological features reliably distinguish SGCs from GCs irrespective of animal age. SGCs maintain higher spontaneous inhibitory postsynaptic current (sIPSC) frequency than GCs from infancy through adulthood. Although sIPSC frequency in SGCs was particularly enhanced during adolescence, sIPSC amplitude and cumulative charge transfer declined from infancy to adulthood and were not different between GCs and SGCs. Extrasynaptic GABA current amplitude peaked in adolescence in both cell types and was significantly greater in SGCs than in GCs only during adolescence. Although GC input resistance was higher than in SGCs during infancy and adolescence, input resistance decreased with developmental age in GCs while it progressively increased in SGCs. Consequently, GCs input resistance was significantly lower than SGCs in adults. The data delineate the structural features that can reliably distinguish GCs from SGCs through development. The results reveal developmental differences in passive membrane properties and steady state inhibition between GCs and SGCs which could confound their use in classifying the cell types.
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影响因子：
5.3
作者：
Gupta, Akshay;Elgammal, Fatima S.;Santhakumar, Vijayalakshmi
通讯作者：
Santhakumar, Vijayalakshmi
影响因子：
2.5
作者：
DUFFY, CJ;RAKIC, P
通讯作者：
RAKIC, P
DOI：
10.1073/pnas.1120754109
发表时间：
2012-03-13
影响因子：
11.1
作者：
Li, Yan;Aimone, James B.;Gage, Fred H.
通讯作者：
Gage, Fred H.
影响因子：
3.4
作者：
Laplagne, Diego A.;Kamienkowski, Juan E.;Schinder, Alejandro F.
通讯作者：
Schinder, Alejandro F.
影响因子：
5.3
作者：
Hatami M;Conrad S;Naghsh P;Alvarez-Bolado G;Skutella T
通讯作者：
Skutella T