Characterization and Expression Profiles of Juvenile Hormone Epoxide Hydrolase From Lymantria dispar (Lepidoptera: Lymantridae) and RNA Interference by Ingestion
Characterization and Expression Profiles of Juvenile Hormone Epoxide Hydrolase From Lymantria dispar (Lepidoptera: Lymantridae) and RNA Interference by Ingestion
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舞毒蛾（鳞翅目：舞毒科）保幼激素环氧化物水解酶的特征和表达谱以及摄入的 RNA 干扰
DOI：
10.1093/jisesa/iey002
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发表时间：
2018-02-08
影响因子：
2.2
通讯作者：
Ma L
中科院分区：
文献类型：
--
作者：
Wen R;Wang B;Wang B;Ma L
保幼激素环氧化物水解酶（JHEH）是昆虫保幼激素（JH）降解途径中的重要酶。它将JH转化为JH二醇，并将JH酸水解为JH酸二醇。JHEH滴度调节昆虫发育的整个过程。本研究从亚洲舞毒蛾Lymantria dispar（L.;鳞翅目：毒蛾科），暂定名为ldjheh 1。LdJHEH 1的特征在于预测的分子量为52.64 kDa，理论等电点为6.87，并且在N-末端含有跨膜结构域。通过qRT-PCR检测ldjheh 1的转录谱。ldjheh 1被发现在整个发育阶段的最大表达水平发生在四龄幼虫。在舞毒蛾三龄幼虫第2天的头部、胸部和腹部检测到ldjheh 1 mRNA。ldjheh 1在3龄幼虫体内也有表达，最高峰出现在蜕皮后24 h。通过口服双链RNA的方法成功敲除了三龄幼虫的ldjheh 1基因。差异在体外产生靶向ldjheh 1的dsRNA。摄取ldjheh 1的dsRNA仅略微延迟幼虫发育。
Juvenile hormone epoxide hydrolase (JHEH) is an important enzyme in the degradation pathways of juvenile hormone (JH) in insects. It converts JH to JH diol and hydrolyses JH acid to JH acid diol. JHEH titers regulate the entire process of insect development. In this study, full length ldjheh cDNA (2101 bp) was cloned from the Asian gypsy moth Lymantria dispar (L.; Lepidoptera: Lymantridae), and provisionally designated ldjheh1. LdJHEH1 was characterized by predicted molecular weight of 52.64 kDa, theoretical isoelectric points of 6.87 and contains a transmembrane domain at the N-terminus. The transcriptional profiles of ldjheh1 were detected by qRT-PCR. The ldjheh1 was found to be expressed throughout all developmental stages with maximum expression levels occurring in fourth instar larvae. The ldjheh1 mRNA was detected in the heads, thoraces, and abdomens of gypsy moth larvae on day 2 of the third instar. The ldjheh1 was also detected in bodies of third instar larvae stage, with the highest peaks occurring at 24 h after ecdysis. The ldjheh1 gene was successfully knocked down by oral delivery dsRNA in the third instar larvae of L. dispar. The dsRNA targeting ldjheh1 was produced in vitro. Ingesting dsRNA for ldjheh1 only slightly delayed larval development.
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DOI：
10.1673/031.008.1301
发表时间：
2008
期刊：
Journal of insect science (Online)
影响因子：
--
作者：
Itakura S;Kawabata S;Tanaka H;Enoki A
通讯作者：
Enoki A
影响因子：
2.2
作者：
Anspaugh, DD;Roe, RM
通讯作者：
Roe, RM
影响因子：
4.8
作者：
Maxwell, RA;Welch, WH;Schooley, DA
通讯作者：
Schooley, DA
影响因子：
2.2
作者：
LASSITER, MT;APPERSON, CS;ROE, RM
通讯作者：
ROE, RM
通过 RNA 干扰表征科罗拉多马铃薯甲虫的两种保幼激素环氧化物水解酶
影响因子：
3.5
作者：
Lu, Feng-Gong;Fu, Kai-Yun;Li, Guo-Qing
通讯作者：
Li, Guo-Qing