Tenascin‐C induction by the diffusible factor epidermal growth factor in stromal‐epithelial interactions
Tenascin‐C induction by the diffusible factor epidermal growth factor in stromal‐epithelial interactions
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基质-上皮相互作用中扩散因子表皮生长因子诱导腱蛋白-C
DOI：
--
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发表时间：
1995
影响因子：
5.6
通讯作者：
M. Saito
中科院分区：
文献类型：
--
作者：
T. Sakai;M. Ohta;Y. Furukawa;Y. Saga;Shinichi Aizawa;H. Kawakatsu;M. Saito
Tenascin-C 是一种六臂细胞外基质糖蛋白，在癌细胞的癌发生和侵袭或转移过程中以时间和空间受限的模式表达，与基质-上皮相互作用相关。人表皮样癌来源的细胞系A431和HEp-2在体外本身不表达生腱蛋白-C，但移植到裸鼠后确实表达生腱蛋白-C，转化生长因子β1（TGF-β1）诱导它们在体外表达生腱蛋白-C。表皮生长因子 (EGF) 比 TGF-β1 更有效地在这些细胞中诱导生腱蛋白-C（大约高 3.5 倍）。肝细胞生长因子（HGF）和血小板衍生生长因子（PDGF）对生腱蛋白-C 的诱导影响不大。 EGF 还诱导其他细胞外基质成分、纤连蛋白和层粘连蛋白。当癌细胞与生腱蛋白-C 基因无效突变纯合小鼠的胚胎成纤维细胞共培养时，或者添加这些细胞的条件培养基时，生腱蛋白-C 也会被诱导。通过用针对 EGF 或其受体的抗体处理细胞，可以减少共培养物中生腱蛋白-C 的诱导。添加 EGF 会导致两种细胞类型破坏其细胞骨架和焦点接触，这一点可以通过应力纤维和纽蛋白斑块的损失来证明。 EGF 既不诱导生腱蛋白-C，也不影响不产生生腱蛋白-C 的 A549 癌细胞的形态，该细胞在移植后不产生生腱蛋白-C。因此，EGF 通过 EGF 受体在不产生生腱蛋白 C 的人癌细胞中诱导生腱蛋白 C。此外，在基质上皮相互作用中，扩散因子 EGF 通过 EGF 受体参与这些细胞中人腱蛋白-C 的诱导。 © 1995 Wiley-Liss Inc.
Tenascin‐C, a six‐armed extracellular matrix glycoprotein, is expressed in a temporally and spatially restricted pattern during carcinogenesis and invasion or metastasis of carcinoma cells in association with stromal‐epithelial interactions. The human epidermoid carcinoma‐derived cell lines, A431 and HEp‐2, which do not express tenascin‐C by themselves in vitro, do express tenascin‐C after transplantation into nude mice, and transforming growth factor β1 (TGF‐β1) induces them to express tenascin‐C in vitro. Epidermal growth factor (EGF) induced tenascin‐C in these cells more effectively (about 3.5‐fold greater) than did TGF‐β1. Hepatocyte growth factor (HGF) and platelet‐derived growth factor (PDGF) had little effect on the induction of tenascin‐C. EGF also induced other extracellular matrix components, fibronectin and laminin. Tenascin‐C was also induced when the carcinoma cells were co‐cultured with embryonic fibroblasts from mice which were homozygous for a null mutation in the tenascin‐C gene, or when the conditioned medium from these cells was added. The induction of tenascin‐C in the co‐culture was reduced by treating the cells with antibodies against EGF or its receptor. The addition of EGF caused both cell types to disrupt their cytoskeleton and focal contacts as evidenced by the loss of stress fibers and vinculin plaques. EGF did neither induce tenascin‐C nor affect the morphology in tenascin‐C‐nonproducing A549 carcinoma cells, which did not produce tenascin‐C after transplantation. Thus, EGF induces tenascin‐C in tenascin‐C‐nonproducing human carcinoma cells through EGF receptors. Furthermore, in stromalepithelial interactions, the diffusible factor EGF participates in the induction of human tenascin‐C in these cells through EGF receptors. © 1995 Wiley‐Liss Inc.
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DOI：
10.1016/s0021-9258(18)48258-0
发表时间：
1987-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ignotz;T. Endo;J. Massagu
通讯作者：
R. Ignotz;T. Endo;J. Massagu
DOI：
--
发表时间：
1986-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ignotz;J. Massagué
通讯作者：
R. Ignotz;J. Massagué
DOI：
10.1177/37.12.2685108
发表时间：
1989-12
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
W. Rettig;P. Garin‐Chesa
通讯作者：
W. Rettig;P. Garin‐Chesa
DOI：
10.1073/pnas.87.17.6497
发表时间：
1990
影响因子：
11.1
作者：
Jones,FS;Crossin,KL;Cunningham,BA;Edelman,GM
通讯作者：
Edelman,GM
转化生长因子-β 会增加正常大鼠肾成纤维细胞中编码表皮生长因子受体和纤连蛋白的基因的转录。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Thompson,KL;Assoian,R;Rosner,MR
通讯作者：
Rosner,MR