Aggressiveness and mycotoxin profile of Fusarium avenaceum isolates causing Fusarium seedling blight and Fusarium head blight in UK malting barley.
Aggressiveness and mycotoxin profile of Fusarium avenaceum isolates causing Fusarium seedling blight and Fusarium head blight in UK malting barley.
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DOI：
10.3389/fpls.2023.1121553
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
燕麦镰刀菌（Fusarium avenaceum）引起大麦上的镰刀菌苗枯病（FSB）和镰刀菌头枯病（FHB），与作物产量和品质的经济损失以及真菌毒素包括恩镰孢菌素（恩斯）A、A1、B和B1的积累相关。虽然F.燕麦是恩斯的主要生产者，但关于分离物在大麦中引起严重镰刀菌病害或产生真菌毒素的能力的研究有限。本研究调查了9株F.燕麦（avenaceum）对两个啤酒大麦品种Moonshine和Quench的ENN真菌毒素谱，并在体外和植物实验中确定了它们的ENN真菌毒素谱。我们评估并比较了由这些分离株引起的FSB和FHB的严重程度与F. graminearum、禾谷镰刀菌F. tricinctum和F. Poae。定量实时聚合酶链反应和液相色谱串联质谱分析被用来量化病原体DNA和霉菌毒素积累，分别在大麦头。F.燕麦对大麦茎和头具有同样的攻击性，并引起最严重的FSB症状，导致茎和根长度减少高达55%。禾谷镰刀菌引起的赤霉病最严重，其次是赤霉病菌。avenaceum的F. avenaceum分离物能够引起与F.燕麦燕麦镰刀菌菌株产生ENN B作为主要的真菌毒素，其次是ENN B1和A1在体外。然而，只有最具攻击性的分离物在植物中产生ENNA A1，并且在植物中或体外都不产生ENNA或白僵菌素（Bevericin，BAE）。F.燕麦分离物产生恩斯的能力与病原体DNA在大麦穗中的积累有关，而FHB的严重程度与ENN A1在植株中的合成和积累有关。CV. Moonshine比Quench对由任何镰刀菌分离物引起的FSB或FHB以及对病原体DNA、恩斯或Escherichia的积累具有更大的抗性。总之，攻击性F.燕麦分离物是引起严重FSB和FHB的有效ENN生产者，ENNA1作为F.燕麦在谷类中。
Fusarium avenaceum causing Fusarium seedling blight (FSB) and Fusarium head blight (FHB) on barley is associated with economic losses of crop yield and quality, and the accumulation of mycotoxins including the enniatins (ENNs) A, A1, B and B1. Although F. avenaceum is the main producer of ENNs, studies on the ability of isolates to cause severe Fusarium diseases or produce mycotoxins in barley are limited. In this work, we investigated the aggressiveness of nine isolates of F. avenaceum to two cultivars of malting barley, Moonshine and Quench, and defined their ENN mycotoxin profiles in in vitro and in planta experiments. We assessed and compared the severity of FSB and FHB caused by these isolates to disease severity by F. graminearum, F. tricinctum and F. poae. Quantitative real-time polymerase chain reaction and Liquid Chromatography Tandem Mass Spectrometry assays were used to quantify pathogen DNA and mycotoxin accumulation, respectively, in barley heads. Isolates of F. avenaceum were equally aggressive to barley stems and heads and caused the most severe FSB symptoms resulting in up to 55% reductions of stem and root length. Fusarium graminearum caused the most severe FHB disease, followed by the isolates of F. avenaceum with the most aggressive F. avenaceum isolates capable of causing similar bleaching of barley heads as F. avenaceum. Fusarium avenaceum isolates produced ENN B as the predominant mycotoxin, followed by ENN B1 and A1 in vitro. However, only the most aggressive isolates produced ENN A1 in planta and none produced ENN A or beauvericin (BEA) either in planta or in vitro. The capacity of F. avenaceum isolates to produce ENNs was related to the accumulation of pathogen DNA in barley heads, whilst FHB severity was related to the synthesis and accumulation of ENN A1 in planta. Cv. Moonshine was significantly more resistant than Quench to FSB or FHB, caused by any Fusarium isolate, and to the accumulation of pathogen DNA, ENNs or BEA. In conclusion, aggressive F. avenaceum isolates are potent ENN producers causing severe FSB and FHB with ENN A1 requiring further investigation as potential virulence factor for F. avenaceum in cereals.
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影响因子：
4.2
作者：
Morcia C;Tumino G;Ghizzoni R;Badeck FW;Lattanzio VM;Pascale M;Terzi V
通讯作者：
Terzi V
影响因子：
3.7
作者：
Eranthodi, Anas;Schneiderman, Danielle;Foroud, Nora A.
通讯作者：
Foroud, Nora A.
DOI：
10.3390/molecules21050627
发表时间：
2016-05-13
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Ferrigo D;Raiola A;Causin R
通讯作者：
Causin R
影响因子：
4.3
作者：
Habler, Katharina;Rychlik, Michael
通讯作者：
Rychlik, Michael
影响因子：
1.8
作者：
Imathiu, Samuel M.;Hare, Martin C.;Edwards, Simon G.
通讯作者：
Edwards, Simon G.