Detection and analysis of bovine foamy virus infection by an indicator cell line
Detection and analysis of bovine foamy virus infection by an indicator cell line
复制标题
指示细胞系检测和分析牛泡沫病毒感染
DOI：
10.1111/j.1745-7254.2007.00563.x
复制
发表时间：
2007-07
影响因子：
8.2
通讯作者：
中科院分区：
文献类型：
--
作者：
摘要：利用牛泡沫病毒（BFV）指示细胞系（BICL）系统，研究BFV在不同培养细胞中的感染性和复制策略。方法：采用共培养法或瞬时转染感染性BFV质粒[巨细胞病毒pCMV - BFV]克隆诱导BFV感染。采用绿色荧光蛋白阳性细胞在BICL中的百分比监测BFV的传染性。在BICL中还评估了逆转录酶抑制剂齐多夫定（AZT）对BFV复制的影响。结果：共培养法在胎牛肺细胞中的BFV滴度比293T和HeLa （Henrietta缺乏细胞）细胞的滴度高4-5倍，同时显著高于在相同细胞中用感染性克隆pCMV-BFV产生的滴度。在病毒感染之前将AZT添加到细胞中，对病毒滴度只有轻微的影响。相反，当病毒在AZT存在的情况下从细胞中产生时，病毒滴度急剧下降到阴性对照的水平。结论：BICL可用于非细胞病变情况下BFV病毒感染的测定。此外，我们提供的重要证据表明，在病毒感染的后期阶段，逆转录对BFV的复制至关重要。
AbstractAim:To determine the infectivity and replication strategy of bovine foamy virus (BFV) in different cultured cells using the BFV indicator cell line (BICL) system.Methods:BFV infection was induced by the co-culture method or the transient transfection of the infectious BFV plasmid [pCMV (cytomegalovirus) - BFV] clone. The infectivity of BFV was monitored by the percentage of green fluorescent protein-positive cells in the BICL. The effect of reverse transcriptase inhibitor zidovudine (AZT) on BFV replication was also evaluated in the BICL.Results:The titer of BFV in fetal bovine lung cells was 4-5-folds more than that in either 293T or HeLa (Cells from Henrietta lacks) cells using the co-culture method, and in the meantime was significantly higher than that produced by the infectious clone pCMV-BFV in the same cells. AZT had only a minor effect on viral titers when added to cells prior to the virus infection. In contrast, viral titers reduced sharply to the level of the negative control when the virus was produced from cells in the presence of AZT.Conclusions:BICL can be used for the titration of the BFV viral infection in non-cytopathic condition. In addition, we provide important evidence to show that reverse transcription is essential for BFV replication at a late step of viral infection.
登录
查看更多内容
影响因子：
--
作者：
M. Löchelt
通讯作者：
M. Löchelt
DOI：
10.1292/jvms.57.65
发表时间：
1995-02
期刊：
The Journal of veterinary medical science
影响因子：
--
作者：
T. Miyazawa;S. Itagaki;K. Tomonaga;Y. Ikeda;T. Mori;Y. Kawaguchi;T. Gemma;Y. Tohya;M. Mochizuki-M.-Moch
通讯作者：
T. Miyazawa;S. Itagaki;K. Tomonaga;Y. Ikeda;T. Mori;Y. Kawaguchi;T. Gemma;Y. Tohya;M. Mochizuki-M.-Moch
影响因子：
5.4
作者：
Delelis, O;Saïb, A;Sonigo, P
通讯作者：
Sonigo, P
影响因子：
3.7
作者：
W. Parks;G. Todaro
通讯作者：
W. Parks;G. Todaro
DOI：
10.1099/0022-1317-44-1-235
发表时间：
1979-07
期刊：
The Journal of general virology
影响因子：
--
作者：
E. Gould;J. Hartley
通讯作者：
E. Gould;J. Hartley