Altered retinol status and expression of retinol-related proteins in streptozotocin-induced type 1 diabetic model rats.
Altered retinol status and expression of retinol-related proteins in streptozotocin-induced type 1 diabetic model rats.
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DOI：
10.3164/jcbn.14-113
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发表时间：
2015-05
影响因子：
2.4
通讯作者：
Tamai H
中科院分区：
文献类型：
--
作者：
Takitani K;Inoue K;Koh M;Miyazaki H;Inoue A;Kishi K;Tamai H
糖尿病是一种以慢性高血糖为特征的代谢紊乱。晚期糖尿病与严重的并发症和营养状况受损有关。在此，我们评估了视黄醇相关蛋白的表达，包括β-胡萝卜素15，15 '-单加氧酶（BCMO），卵磷脂：视黄醇酰基转移酶（LRAT），醛脱氢酶（ALDH）和细胞色素P450 26 A1（CYP 26 A1），并测量了链脲佐菌素（STZ）诱导的1型糖尿病模型大鼠血浆和肝脏中的视黄醇水平。与对照组大鼠相比，STZ大鼠血浆和肝脏中的视黄醇水平分别降低和升高。STZ大鼠肝脏LRAT基因表达低于对照组。在STZ大鼠的肝脏中，ALDH 1A 1，视网膜代谢酶的表达较高，而ALDH 1A 2的表达低于对照组。STZ组大鼠肝脏CYP 26 A1表达明显高于对照组。STZ组大鼠肝、肠组织中BCMO表达水平明显低于对照组。BCMO表达改变可能影响视黄醇状态。据认为，尽管视黄醇的加速催化，但视黄醇的代谢利用率降低;因此，1型糖尿病状态下大鼠肝脏中的视黄醇动员可能不平衡。
Diabetes is a metabolic disorder characterized by chronic hyperglycemia. Advanced diabetes is associated with severe complications and impaired nutritional status. Here, we assessed the expression of retinol-associated proteins, including β-carotene 15,15'-monooxygenase (BCMO), lecithin:retinol acyltransferase (LRAT), aldehyde dehydrogenase (ALDH), and cytochrome P450 26A1 (CYP26A1), and measured retinol levels in the plasma and liver of streptozotocin (STZ)-induced type 1 diabetic model rats. Compared to the levels in the control rats, retinol levels in the plasma and liver of STZ rats were decreased and increased, respectively. Hepatic expression of the LRAT gene in STZ rats was lower than that in the controls. In the liver of STZ rats, the expression of ALDH1A1, a retinal metabolizing enzyme was higher, whereas ALDH1A2 expression was lower than in the controls. Hepatic CYP26A1 expression in STZ rats was significantly higher than in the control rats. BCMO expression levels in the liver and intestine of STZ rats were much lower than those of the controls. Altered BCMO expression might affect retinol status. It is considered that the metabolic availability of retinol was lessened despite the accelerated catabolism of retinol; therefore, retinol mobilization may be unbalanced in the liver of rats in the type 1 diabetic state.
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影响因子：
6
作者：
Granado, F;Olmedilla, B;Rojas-Hidalgo, E
通讯作者：
Rojas-Hidalgo, E
影响因子：
1.6
作者：
Morinobu, T;Tamai, H
通讯作者：
Tamai, H
影响因子：
4.8
作者：
Batten, ML;Imanishi, Y;Palczewski, K
通讯作者：
Palczewski, K
影响因子：
1.6
作者：
Miyazaki, Hiroshi;Takitani, Kimitaka;Tamai, Hiroshi
通讯作者：
Tamai, Hiroshi
影响因子：
2.7
作者：
Samarghandian, Saeed;Borji, Abasalt;Samini, Fariborz
通讯作者：
Samini, Fariborz