Neural induction and regionalisation in the chick embryo.
Neural induction and regionalisation in the chick embryo.
复制标题
鸡胚胎中的神经诱导和区域化。
作者：
Kg Storey;Jm Crossley;Em Derobertis;We Norris;Cd Stern
通过将 Hensen 结移植到宿主胚胎的胚胎外区域（混浊边缘区域）来研究小鸡神经系统的诱导和区域化。使用小鸡/鹌鹑嵌合体确定宿主和供体组织对多生轴的贡献，并使用三种分子标记：Engrailed、神经丝（抗体 3A10）和 XlHbox1/Hox3.3 来帮助识别异位轴的特定区域。我们发现，节点的年龄决定了形成神经系统的区域：年轻的节点（2-4 阶段）诱导前神经系统和后神经系统，而较老的节点（5-6 阶段）诱导能力降低，仅生成后神经系统。通过改变宿主胚胎的年龄，我们发现外胚层对神经诱导作出反应的能力在第4阶段之后下降。我们的结论是，在正常发育过程中，神经诱导的初始步骤发生在第4阶段之前，神经系统的前后区域化可能是一个较晚的过程，可能与脊索的分化有关。我们还推测，负责诱导头部中枢神经系统的机制与产生脊髓的机制不同：躯干中枢神经系统可能是通过前部中枢神经系统的同源诱导或通过非常早期诱导的神经原基的伸长而产生的。
Induction and regionalisation of the chick nervous system were investigated by transplanting Hensen's node into the extra-embryonic region (area opaca margin) of a host embryo. Chick/quail chimaeras were used to determine the contributions of host and donor tissue to the supernumerary axis, and three molecular markers, Engrailed, neurofilaments (antibody 3A10) and XlHbox1/Hox3.3 were used to aid the identification of particular regions of the ectopic axis. We find that the age of the node determines the regions of the nervous system that form: young nodes (stages 2-4) induced both anterior and posterior nervous system, while older nodes (stages 5-6) have reduced inducing ability and generate only posterior nervous system. By varying the age of the host embryo, we show that the competence of the epiblast to respond to neural induction declines after stage 4. We conclude that during normal development, the initial steps of neural induction take place before stage 4 and that anteroposterior regionalisation of the nervous system may be a later process, perhaps associated with the differentiating notochord. We also speculate that the mechanisms responsible for induction of head CNS differ from those that generate the spinal cord: the trunk CNS could arise by homeogenetic induction by anterior CNS or by elongation of neural primordia that are induced very early.
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Lbx1 表达和青蛙肢体发育。
影响因子：
2.4
作者：
Sabo,MichelleC;Nath,Kimberly;Elinson,RichardP
通讯作者：
Elinson,RichardP
改进了小鸡-鹌鹑嵌合体的组织学。
影响因子：
--
作者：
Hutson,JM;Donahoe,PK
通讯作者：
Donahoe,PK
影响因子：
4.6
作者：
Anders Molven;Christopher V. E. Wright;R. Bremiller;E. M. D. Robertis;Charles B. Kimmel
通讯作者：
Anders Molven;Christopher V. E. Wright;R. Bremiller;E. M. D. Robertis;Charles B. Kimmel
非洲爪蟾前脊索对嵌入蛋白的区域特异性神经诱导。
DOI：
10.1126/science.1978411
发表时间：
1990
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hemmati-Brivanlou,A;Stewart,RM;Harland,RM
通讯作者：
Harland,RM