Effects of dietary vitamin D3 on growth performance, antioxidant capacities and innate immune responses in juvenile black carp Mylopharyngodon piceus
Effects of dietary vitamin D3 on growth performance, antioxidant capacities and innate immune responses in juvenile black carp Mylopharyngodon piceus
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膳食维生素 D3 对青鱼幼鱼生长性能、抗氧化能力和先天免疫反应的影响
DOI：
10.1007/s10695-020-00876-8
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发表时间：
2020-09
影响因子：
2.9
通讯作者：
Jinyun Ye
中科院分区：
文献类型：
--
作者：
Chenglong Wu;Bin Lu;Yunlong Wang;Chuanmin Jin;Yixiang Zhang;Jinyun Ye
本试验旨在研究饲料中不同维生素D3含量对青鱼幼鱼生长性能、抗氧化能力和天然免疫功能的影响。在饲料中分别添加96、220、412、840、1480和3008 IU/Kg的维生素D3（VD 3），试验期为9周。结果表明，根据二次多项式回归模型，在534.2IU/Kg饲粮VD 3水平下，增重和特殊生长率最高。添加412、840和1480 IU/Kg的维生素D3可显著提高青鱼的蛋白质效率（p < 0.05），降低饲料转化率（p < 0.05）。饲料中维生素D3含量（412、840和1480 IU/Kg）可显著上调青鱼肝脏中脂氧合酶5（LPO 5）的表达水平，提高超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPX）和谷胱甘肽还原酶（GR）的活性，提高GSH含量和总抗氧化能力（T-AOC）。然而，谷胱甘肽S-转移酶（GST）活性和丙二醛（MDA）水平显着降低饲料中维生素D3含量（412，840，1480 IU/Kg）在鱼的肝脏。与维生素D3缺乏组（96 IU/Kg）相比，412、840和1480 IU/Kg维生素D3可显著上调青鱼幼鱼血细胞和肝脏中α-干扰素（IFN-α）、溶菌酶（LYZ）、铁调素（HEPC）、天然抗性相关巨噬细胞蛋白（NRAMP）、补体成分3（C3）和C9的mRNA表达水平。同时，高含量的维生素D3能提高青鱼幼鱼血清LYZ和ACP活性以及C3和C4含量。这些结果表明，饲料中添加适量的维生素D3可以提高青鱼幼鱼的生长性能，提高其抗氧化能力，进而提高其先天免疫指标。
The aim of this experiment was used to investigate the effects of different contents of dietary vitamin D3on the growth performance and antioxidant and innate immune responses in juvenile black carp Mylopharyngodon piceus. Black carp juveniles were fed six levels of dietary vitamin D3(VD3) (96, 220, 412, 840, 1480, and 3008 IU/Kg) for 9 weeks. Results showed that highest weight gain (WG) and special growth ratio (SGR) were obtained at 534.2 IU/Kg dietary VD3according to the second-order polynomial regression model. The protein efficiency ratio (PER) of black carp could be significantly increased by 412, 840, and 1480 IU/Kg dietary VD3(p < 0.05), while the feed conversion ratio (FCR) were reduced by 412, 840, and 1480 IU/Kg dietary VD3(p < 0.05). Adequate dietary VD3content (412, 840, and 1480 IU/Kg) could significantly upregulate expression levels of lipoxygenase 5 (LPO 5); increase the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GR); and improve GSH contents and total antioxidant capacities (T-AOC) in the liver of black carp. However, glutathione S-transferase (GST) activities and malondialdehyde (MDA) levels were significantly reduced by adequate dietary VD3content (412, 840, and 1480 IU/Kg) in the fish liver. In addition, 412, 840, and 1480 IU/Kg dietary VD3could significantly upregulate the mRNA expression levels of interferon-α (IFN-α), lysozyme (LYZ), hepcidin (HEPC), natural resistance-associated macrophage protein (NRAMP), and complement component 3 (C3) and C9 in the hemocytes and liver of black carp juveniles compared with the VD3-deficient diet (96 IU/Kg). Meanwhile, higher contents of dietary VD3could increase serum LYZ and ACP activities and C3 and C4 contents in black carp juveniles compared with the groups fed VD3-deficient diet. In conclusion, these results suggest that adequate dietary VD3could increase growth performances, improve antioxidant capacities, and then enhance innate immune parameters in black carp juveniles.
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影响因子：
10.2
作者：
Michael J. Thomas
通讯作者：
Michael J. Thomas
影响因子：
3.5
作者：
H. Wiseman
通讯作者：
H. Wiseman
影响因子：
4.5
作者：
B. Woodward
通讯作者：
B. Woodward
DOI：
10.1172/jci15770
发表时间：
2002-05
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
T. Decker;Silvia Stockinger;Marina Karaghiosoff;Mathias Müller;P. Kovarik
通讯作者：
T. Decker;Silvia Stockinger;Marina Karaghiosoff;Mathias Müller;P. Kovarik
影响因子：
4.5
作者：
Miao Linghong;Ge Xianping;Xie Jun;L. Bo;Wang Ke-bao;Zhu Jian;Ren Ming-chun;Zhou Qun-lan;Pan Liangkun;Chen Ruli
通讯作者：
Miao Linghong;Ge Xianping;Xie Jun;L. Bo;Wang Ke-bao;Zhu Jian;Ren Ming-chun;Zhou Qun-lan;Pan Liangkun;Chen Ruli