Pro-inflammatory mediation of myoblast proliferation.
Pro-inflammatory mediation of myoblast proliferation.
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DOI：
10.1371/journal.pone.0092363
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lees SJ
中科院分区：
文献类型：
--
作者：
Otis JS;Niccoli S;Hawdon N;Sarvas JL;Frye MA;Chicco AJ;Lees SJ
骨骼肌卫星细胞的功能在很大程度上取决于损伤后的周围环境。免疫细胞浸润在受伤区域的细胞外空间占主导地位，导致细胞因子浓度增加。虽然先前已经在损伤后的前3天建立了促炎性细胞因子表达增加，但对细胞因子表达的时间过程和细胞因子诱导的成肌细胞功能的具体机制知之甚少。因此，在损伤后的几个时间点检测IL-1β和IL-6的表达，以及它们对成肌细胞增殖的影响。为了做到这一点，使用氯化钡损伤小鼠的骨骼肌，并在损伤后1、5、10和28天收集组织。细胞因子诱导的增殖的机制在细胞培养中使用原代成肌细胞和C2 C12成肌细胞来确定。发现在损伤后5天，IL-1β（p≤0.05）和IL-6（p = 0.06）表达增加了20倍。 IL-1β使原代和C2 C12细胞的增殖均增加约25%。IL-1β刺激还导致NF-κB活性增加，可能有助于增殖增加。这些数据首次证明，单独使用IL-1β可以增加原代骨骼肌卫星细胞的促有丝分裂活性，并提供了对损伤后决定卫星细胞功能的机制的深入了解。
Skeletal muscle satellite cell function is largely dictated by the surrounding environment following injury. Immune cell infiltration dominates the extracellular space in the injured area, resulting in increased cytokine concentrations. While increased pro-inflammatory cytokine expression has been previously established in the first 3 days following injury, less is known about the time course of cytokine expression and the specific mechanisms of cytokine induced myoblast function. Therefore, the expression of IL-1β and IL-6 at several time points following injury, and their effects on myoblast proliferation, were examined. In order to do this, skeletal muscle was injured using barium chloride in mice and tissue was collected 1, 5, 10, and 28 days following injury. Mechanisms of cytokine induced proliferation were determined in cell culture using both primary and C2C12 myoblasts. It was found that there is a ∼20-fold increase in IL-1β (p≤0.05) and IL-6 (p = 0.06) expression 5 days following injury. IL-1β increased proliferation of both primary and C2C12 cells ∼25%. IL-1β stimulation also resulted in increased NF-κB activity, likely contributing to the increased proliferation. These data demonstrate for the first time that IL-1β alone can increase the mitogenic activity of primary skeletal muscle satellite cells and offer insight into the mechanisms dictating satellite cell function following injury.
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DOI：
10.1084/jem.20052367
发表时间：
2006-04-17
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Cumberbatch M;Singh M;Dearman RJ;Young HS;Kimber I;Griffiths CE
通讯作者：
Griffiths CE
影响因子：
11.8
作者：
Griffin CA;Kafadar KA;Pavlath GK
通讯作者：
Pavlath GK
影响因子：
2.2
作者：
Clary CR;Guidot DM;Bratina MA;Otis JS
通讯作者：
Otis JS
影响因子：
--
作者：
FIELDING, RA;MANFREDI, TJ;CANNON, JG
通讯作者：
CANNON, JG
DOI：
10.1152/ajpregu.00039.2002
发表时间：
2002-09-01
影响因子：
2.8
作者：
Frost, RA;Nystrom, GJ;Lang, CH
通讯作者：
Lang, CH