New trends of HCV infection in China revealed by genetic analysis of viral sequences determined from first-time volunteer blood donors.
New trends of HCV infection in China revealed by genetic analysis of viral sequences determined from first-time volunteer blood donors.
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对首次无偿献血者病毒序列进行基因分析，揭示中国HCV感染新趋势。
DOI：
10.1111/j.1365-2893.2010.01280.x
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发表时间：
2011-01
影响因子：
2.5
通讯作者：
Nelson K
中科院分区：
文献类型：
--
作者：
Fu Y;Wang Y;Xia W;Pybus OG;Qin W;Lu L;Nelson K
最近，我们研究了中国559890名首次志愿献血者的丙型肝炎病毒（HCV）血清患病率。从随机选择的450名抗HCV阳性供者中，我们检测了270名供者的HCV RNA。在这项研究中，我们从236名供者中扩增了HCV E1和/或NS5B序列，然后进行了DNA测序和系统发育分析。结果提示了中国HCV感染的新趋势。HCV基因型分布因供者的原籍地区而异。在广东省献血者中，6a、1b、3a、3b、2a和1a亚型的检出率分别为49.7%、31.0%、7.6%、5.5%、4.1%和2.1%。在广东省以外的献血者中，1b、2a、6a、3b、3a、6e和6n的检出率分别为57.1%、13.2%、11.0%、9.9%、4.4%、2.2%和2.2%。虽然我们发现不同地区在年龄和性别上没有显著差异，但广东供体中6a亚型比其他地区更常见（P< 0.001），而1b亚型（P< 0.02）和2a亚型（P< 0.001）在广东以外地区更常见。不考虑来源，6a亚型感染供者的男女比例高于1b亚型供者（P < 0.05），而2a亚型供者的平均年龄比所有其他亚型的年龄大8-10岁（P < 0.05）。对我们的序列数据进行详细的系统发育分析，可以进一步了解HCV在中国境内以及中国与其他国家之间的传播。HCV 6a在广东献血者中的优势是惊人的，这需要对其获得的危险因素进行研究。
Recently, we studied hepatitis C virus (HCV) sera-prevalence among 559 890 first-time volunteer blood donors in China. From randomly selected 450 anti-HCV positive donors, we detected HCV RNA in 270 donors. In this study, we amplified HCV E1 and/or NS5B sequences from 236 of these donors followed by DNA sequencing and phylogenetic analysis. The results indicate new trends of HCV infection in China. The HCV genotype distribution differed according to the donors’ region of origin. Among donors from Guangdong province, we detected subtypes 6a, 1b, 3a, 3b, 2a, and 1a at frequencies of 49.7%, 31.0%, 7.6%, 5.5%, 4.1%, and 2.1%, respectively. Among donors from outside Guangdong, we detected 1b, 2a, 6a, 3b, 3a, 6e, and 6n at frequencies 57.1%, 13.2%, 11.0%, 9.9%, 4.4%, 2.2%, and 2.2%, respectively. Although we found no significant differences among regions in age or gender, subtype 6a was more common (P< 0.001) in donors from Guangdong than those from elsewhere, whilst subtypes 1b (P< 0.02) and 2a (P < 0.001) were more frequent outside Guangdong. Disregarding origins, the male/female ratio was higher for subtype 6a-infected donors (P < 0.05) than for subtype 1b donors, whilst the mean age of subtype 2a donors was 8–10 years older (P < 0.05) than that for all other subtypes. Detailed phylogenetic analysis of our sequence data provides further insight into the transmission of HCV within China, and between China and other countries. The predominance of HCV 6a among blood donors in Guangdong is striking and mandates studies into risk factors for its acquisition.
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影响因子：
1.5
作者：
Chang, Sui-Yuan;Sheng, Wang-Huei;Chang, Shan-Chwen
通讯作者：
Chang, Shan-Chwen
影响因子：
13.5
作者：
Murphy, EL;Bryzman, SM;Nemo, GJ
通讯作者：
Nemo, GJ
影响因子：
11.8
作者：
Garten, RJ;Zhang, JB;Yu, XF
通讯作者：
Yu, XF
影响因子：
168.9
作者：
Shan, H;Wang, JX;Ness, PM
通讯作者：
Ness, PM
影响因子：
13.5
作者：
Hoofnagle, JH
通讯作者：
Hoofnagle, JH