Management of hepatitis E virus (HEV) zoonotic transmission: protection of rabbits against HEV challenge following immunization with HEV 239 vaccine.
Management of hepatitis E virus (HEV) zoonotic transmission: protection of rabbits against HEV challenge following immunization with HEV 239 vaccine.
复制标题
戊型肝炎病毒 (HEV) 人畜共患传播的管理：使用 HEV 239 疫苗免疫后保护兔子免受 HEV 挑战
DOI：
10.1371/journal.pone.0087600
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhuang H
中科院分区：
文献类型：
--
作者：
Liu P;Du Rj;Wang L;Han J;Liu L;Zhang Yl;Xia Jk;Lu Fm;Zhuang H
戊型肝炎病毒（HEV）构成了世界范围内的重大健康负担，估计约有33%的世界人口暴露于该病原体。我国最近批准的HEV239疫苗在一般人群和孕妇中对基因1型和基因4型HEV显示出良好的保护效果。由于戊型肝炎是一种人畜共患病，因此还需要确定该疫苗是否可以用于管理人类HEV感染的动物源。为了测试HEV 239疫苗在保护动物HEV储库免受HEV感染方面的功效，将12只无特异性病原体（SPF）的兔随机分成两组，每组6只动物，并肌内接种HEV 239和安慰剂（PBS）。所有动物在初次免疫后7周用基因型4的猪HEV或兔HEV静脉内攻击。通过血清ALT水平、病毒血症持续时间、粪便病毒排泄和HEV抗体应答监测感染过程10周。所有用HEV 239免疫的兔均产生高滴度的抗HEV，并且在整个实验中未观察到HEV感染的迹象，而用PBS接种的兔在攻击后产生病毒性肝炎，伴随肝酶升高、病毒血症和粪便病毒脱落。结果表明，HEV239疫苗对家兔具有高度免疫原性，能完全保护家兔抵抗同种和异种HEV感染。这些发现有助于在发展中国家和工业化国家预防食源性散发性HEV感染。
Hepatitis E virus (HEV) constitutes a significant health burden worldwide, with an estimated approximately 33% of the world’s population exposed to the pathogen. The recent licensed HEV 239 vaccine in China showed excellent protective efficacy against HEV of genotypes 1 and 4 in the general population and pregnant women. Because hepatitis E is a zoonosis, it is also necessary to ascertain whether this vaccine can serve to manage animal sources of human HEV infection. To test the efficacy of the HEV 239 vaccine in protecting animal reservoirs of HEV against HEV infection, twelve specific-pathogen-free (SPF) rabbits were divided randomly into two groups of 6 animals and inoculated intramuscularly with HEV 239 and placebo (PBS). All animals were challenged intravenously with swine HEV of genotype 4 or rabbit HEV seven weeks after the initial immunization. The course of infection was monitored for 10 weeks by serum ALT levels, duration of viremia and fecal virus excretion and HEV antibody responses. All rabbits immunized with HEV 239 developed high titers of anti-HEV and no signs of HEV infection were observed throughout the experiment, while rabbits inoculated with PBS developed viral hepatitis following challenge, with liver enzyme elevations, viremia, and fecal virus shedding. Our data indicated that the HEV 239 vaccine is highly immunogenic for rabbits and that it can completely protect rabbits against homologous and heterologous HEV infections. These findings could facilitate the prevention of food-borne sporadic HEV infection in both developing and industrialized countries.
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影响因子：
11.8
作者：
Li TC;Chijiwa K;Sera N;Ishibashi T;Etoh Y;Shinohara Y;Kurata Y;Ishida M;Sakamoto S;Takeda N;Miyamura T
通讯作者：
Miyamura T
影响因子：
11.8
作者：
Liu P;Bu QN;Wang L;Han J;Du RJ;Lei YX;Ouyang YQ;Li J;Zhu YH;Lu FM;Zhuang H
通讯作者：
Zhuang H
影响因子：
3.2
作者：
Geng, Yansheng;Zhao, Chenyan;Wang, Youchun
通讯作者：
Wang, Youchun
影响因子：
9.4
作者：
Meng, XJ;Wiseman, B;Purcell, RH
通讯作者：
Purcell, RH
影响因子：
158.5
作者：
Kamar, Nassim;Selves, Janick;Rostaing, Lionel
通讯作者：
Rostaing, Lionel