Intra-aortic clusters undergo endothelial to hematopoietic phenotypic transition during early embryogenesis.
Intra-aortic clusters undergo endothelial to hematopoietic phenotypic transition during early embryogenesis.
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DOI：
10.1371/journal.pone.0035763
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Sugiyama D
中科院分区：
文献类型：
--
作者：
Mizuochi C;Fraser ST;Biasch K;Horio Y;Kikushige Y;Tani K;Akashi K;Tavian M;Sugiyama D
主动脉内簇（IAC）附着在大动脉底部，被认为最近在脊椎动物妊娠中期胚胎中获得了造血干细胞（HSC）潜力。人们对 IAC 的形成和功能知之甚少。为了解决这个问题，通过免疫组织化学和流式细胞术对小鼠胚胎中的 IAC 进行了表征。免疫组织化学分析显示IAC同时表达表面抗原CD31、CD34和c-Kit。当胚胎从 9.5 dpc 发育到 10.5 dpc 时，IAC 上调造血标志物 CD41 和 CD45，同时下调内皮表面抗原 VE-cadherin/CD144，表明 IAC 在 9.5 dpc 后失去内皮表型。 IAC 的造血潜力分析显示巨噬细胞 CFC 活性从 9.5 dpc 到 10.5 dpc 发生显着变化。为了进一步表征 IAC，我们根据 CD45 表达分离了 IAC。相应地，IACs CD45(neg)部分中造血转录因子的表达显着上调。这些结果表明，IAC 从内皮表型向造血表型的转变发生在 9.5 dpc 后。
Intra-aortic clusters (IACs) attach to floor of large arteries and are considered to have recently acquired hematopoietic stem cell (HSC)-potential in vertebrate early mid-gestation embryos. The formation and function of IACs is poorly understood. To address this issue, IACs were characterized by immunohistochemistry and flow cytometry in mouse embryos. Immunohistochemical analysis revealed that IACs simultaneously express the surface antigens CD31, CD34 and c-Kit. As embryos developed from 9.5 to 10.5 dpc, IACs up-regulate the hematopoietic markers CD41 and CD45 while down-regulating the endothelial surface antigen VE-cadherin/CD144, suggesting that IACs lose endothelial phenotype after 9.5 dpc. Analysis of the hematopoietic potential of IACs revealed a significant change in macrophage CFC activity from 9.5 to 10.5 dpc. To further characterize IACs, we isolated IACs based on CD45 expression. Correspondingly, the expression of hematopoietic transcription factors in the CD45(neg) fraction of IACs was significantly up-regulated. These results suggest that the transition from endothelial to hematopoietic phenotype of IACs occurs after 9.5 dpc.
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影响因子：
64.8
作者：
Chen, Michael J.;Yokomizo, Tomomasa;Zeigler, Brandon M.;Dzierzak, Elaine;Speck, Nancy A.
通讯作者：
Speck, Nancy A.
AGM地区发展中HSC谱系的分层组织和早期造血规范。
DOI：
10.1084/jem.20102419
发表时间：
2011-06-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Rybtsov S;Sobiesiak M;Taoudi S;Souilhol C;Senserrich J;Liakhovitskaia A;Ivanovs A;Frampton J;Zhao S;Medvinsky A
通讯作者：
Medvinsky A
内素蛋白是一种CD34样唾液素，在整个发育过程中都标志着造血干细胞。
DOI：
10.1084/jem.20051325
发表时间：
2005-12-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
--
影响因子：
0.7
作者：
Jaffredo, T;Bollerot, K;Drevon, C
通讯作者：
Drevon, C
影响因子：
20.3
作者：
McKinney-Freeman, Shannon L.;Naveiras, Olaia;Daley, George Q.
通讯作者：
Daley, George Q.