Molecular characterization of the microbial flora residing at the apical portion of infected root canals of human teeth.
Molecular characterization of the microbial flora residing at the apical portion of infected root canals of human teeth.
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DOI：
10.1016/j.joen.2011.06.020
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发表时间：
2011-10
影响因子：
4.2
通讯作者：
Lux R
中科院分区：
文献类型：
--
作者：
Chugal N;Wang JK;Wang R;He X;Kang M;Li J;Zhou X;Shi W;Lux R
本研究采用独立于培养的分子生物学方法，研究了原发性和继发性感染中患有根尖周炎的人类牙齿根尖部的细菌群落。拔牙根尖根段的根管样本是从 18 颗牙髓坏死的牙齿和 8 颗之前接受过牙髓治疗的牙齿中采集的。样品通过聚合酶链式反应 (PCR) 进行扩增，并通过变性梯度凝胶电泳 (DGGE) 进行分离。从凝胶中切下选定的条带并进行测序以进行鉴定。与之前对整个根管的研究相比，原发感染中的根尖细菌群落明显比继发感染中的细菌群落更加多样化（p=0.0003）。患者间和患者内部的比较显示出相似的概况变化。继发感染的同一颗牙齿的不同牙根在细菌组成上表现出较低的相似性，而从原发感染中收集的等效样本包含几乎相同的菌群。测序显示梭杆菌、放线菌属的患病率很高。和口腔厌氧球菌两种类型的感染。许多继发感染含有伯克霍尔德氏菌目或假单胞菌属。两者都代表机会性环境病原体。某些微生物在原发性和继发性感染中表现出相似的患病率，表明它们可能在牙髓治疗过程中未被根除。伯克霍尔德氏菌目和假单胞菌属的存在。凸显环境污染问题。治疗似乎对多根牙的各个根管产生不同的影响，导致局部微生物群的变化。
This study investigated the bacterial communities residing in the apical portion of human teeth with apical periodontitis in primary and secondary infections using a culture-independent molecular biology approach. Root canal samples from the apical root segments of extracted teeth were collected from 18 teeth with necrotic pulp and 8 teeth with previous endodontic treatment. Samples were processed for amplification via polymerase chain reaction (PCR) and separated with denaturing gradient gel electrophoresis (DGGE). Selected bands were excised from the gel and sequenced for identification. Comparable to previous studies of entire root canals, the apical bacterial communities in primary infections were significantly more diverse than in secondary infections (p=0.0003). Inter- and intra-patient comparisons exhibited similar variations in profiles. Different roots of the same teeth with secondary infections displayed low similarity in bacterial composition, while an equivalent sample collected from primary infection contained almost identical populations. Sequencing revealed a high prevalence of fusobacteria, Actinomyces sp. and oral Anaeroglobus geminatus in both types of infection. Many secondary infections contained Burkholderiales or Pseudomonas sp. both of which represent opportunistic environmental pathogens. Certain microorganisms exhibit similar prevalence in primary and secondary infection indicating that they are likely not eradicated during endodontic treatment. The presence of Burkholderiales and Pseudomonas sp. underscores the problem of environmental contamination. Treatment appears to affect the various root canals of multi-rooted teeth differently, resulting in local changes of the microbiota.
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DOI：
10.1016/j.tripleo.2009.01.042
发表时间：
2009-05-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Siqueira, Jose F., Jr.;Rocas, Isabela N.;Silva, Marlei G.
通讯作者：
Silva, Marlei G.
影响因子：
4.2
作者：
Alves, Flavio R. F.;Siqueira, Jose F., Jr.;Rosado, Alexandre S.
通讯作者：
Rosado, Alexandre S.
影响因子：
7.6
作者：
Siqueira, J. F., Jr.;Rocas, I. N.
通讯作者：
Rocas, I. N.
影响因子：
--
作者：
Sakamoto, M.;Siqueira, J. F., Jr.;Benno, Y.
通讯作者：
Benno, Y.
DOI：
10.1016/j.tripleo.2009.05.028
发表时间：
2009-10-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Ricucci, Domenico;Lin, Louis M.;Spangberg, Larz S. W.
通讯作者：
Spangberg, Larz S. W.