Oral dysplasia and squamous cell carcinoma: correlation between increased expression of CD21, Epstein-Barr virus and CK19.
Oral dysplasia and squamous cell carcinoma: correlation between increased expression of CD21, Epstein-Barr virus and CK19.
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DOI：
10.1016/j.oraloncology.2012.03.017
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发表时间：
2012-09
期刊：
影响因子：
4.8
通讯作者：
Hutt-Fletcher, Lindsey M.
中科院分区：
文献类型：
--
作者：
Jiang, Ru;Gu, Xin;Moore-Medlin, Tara N.;Nathan, Cherie-Ann;Hutt-Fletcher, Lindsey M.
EB病毒是一种经口传播的人γ疱疹病毒，感染B淋巴细胞和上皮细胞。虽然大多数原发性感染是无症状的，但病毒的长期携带可能与淋巴或上皮恶性肿瘤有关。EB病毒与口腔鳞状细胞癌的关联是散发的，并且不确定病毒是否参与肿瘤的起始或可能参与其进展。补体受体2型，CR2或CD21，是EBV的主要附着蛋白的受体，其显著增强上皮细胞感染，但其在正常组织上的表达限于扁桃体和腺样上皮。据报道，随着细胞发育不良，它们表达更高水平的CK19。我们试图评估CD21和CK19表达是否随着Waldeyer环外的口腔上皮细胞发育不良而变化。通过激光捕获显微切割分离上皮细胞，并通过定量逆转录酶PCR测量CD 21、CK 19和EB病毒RNA的水平。我们报告说，CD21的表达增加的频率和强度，口腔上皮细胞变得更加异型增生和表达与EBV感染的增加。携带EBV的肿瘤或发育不良病变通常也比不携带EBV的肿瘤或发育不良病变表达更高水平的CK19。研究结果表明，发育异常可能使细胞更容易受到EB病毒感染，并且病毒感染可能会改变受感染细胞的表型，从而影响预后。
Epstein-Barr virus is an orally transmitted human gammaherpesvirus that infects B lymphocytes and epithelial cells. Although most primary infections are asymptomatic, long term carriage of the virus can be associated with either lymphoid or epithelial malignancies. The association of EBV with oral squamous cell carcinomas is sporadic and it is uncertain if the virus is involved in initiation of the tumor or, possibly, in its progression. Complement receptor type 2, CR2 or CD21, is a receptor for the major attachment protein of EBV, which significantly enhances epithelial cell infection, but its expression on normal tissues is restricted to tonsil and adenoid epithelium. As cells become dysplastic they are reported to express higher levels of CK19. We sought to evaluate whether CD21 and CK19 expression change as oral epithelial cells outside Waldeyer’s ring become dysplastic. Epithelial cells were isolated by laser capture microdissection and levels of CD21, CK19 and EBV RNA were measured by quantitative reverse transcriptase PCR. We report that expression of CD21 increases in frequency and intensity as oral epithelial cells become more dysplastic and that expression correlates with an increase in infection by EBV. Tumors or dysplastic lesions that carry EBV also generally express higher levels of CK19 than those that do not. The findings suggest that dysplasia may make cells more susceptible to infection by EBV and that infection by the virus may alter the phenotype of the infected cell in a manner which could affect prognosis.
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DOI：
10.1073/pnas.0907508106
发表时间：
2009-12-01
影响因子：
11.1
作者：
Chesnokova, Liudmila S.;Nishimura, Stephen L.;Hutt-Fletcher, Lindsey M.
通讯作者：
Hutt-Fletcher, Lindsey M.
影响因子：
2.1
作者：
Safadi, Rima A.;Musleh, Atika S.;Hamasha, Abed Al-Hadi
通讯作者：
Hamasha, Abed Al-Hadi
影响因子：
6.7
作者：
Hadinoto V;Shapiro M;Sun CC;Thorley-Lawson DA
通讯作者：
Thorley-Lawson DA
影响因子：
10.3
作者：
Chen, S.;Takahara, M.;Furue, M.
通讯作者：
Furue, M.
影响因子：
5.4
作者：
Borza, CM;Morgan, AJ;Hutt-Fletcher, LM
通讯作者：
Hutt-Fletcher, LM