Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy: a cross-validation with in vitro measurements.
Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy: a cross-validation with in vitro measurements.
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DOI：
10.1002/jmri.22733
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发表时间：
2011-11
影响因子：
4.4
通讯作者：
Port, John D.
中科院分区：
文献类型：
--
作者：
Lanza, Ian R.;Bhagra, Sumit;Nair, K. Sreekumaran;Port, John D.
关键词：
交叉验证31 P-MRS测量的骨骼肌氧化能力与18名健康成人相同肌群肌肉活检组织中分离的线粒体体外氧化能力测量值。体内氧化能力由30 s最大等长膝关节伸展后的PCr恢复动力学确定。使用高分辨率呼吸测定法在分离的线粒体中测量状态3呼吸。第二组10个人进行了两次31 P-MRS检测，以评估该方法的重复检测重现性。总体而言，体内和体外方法相关性良好（r = 0.66 - 0.72），Bland Altman图显示出良好的一致性。观察到PCr回收率常数（CV = 4.6%，ICC = 0.85）和计算的氧化能力（CV = 3.4%，ICC = 0.83）具有良好的重现性。这些结果表明，31 P-MRS符合金标准体外测量，是高度可重复的。
To cross-validate skeletal muscle oxidative capacity measured by 31P-MRS with in vitro measurements of oxidative capacityin mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults. Oxidative capacity in vivo was determined from PCr recovery kinetics following a 30s maximal isometric knee extension. State 3 respiration was measured in isolated mitochondria using high-resolution respirometry. A second cohort of 10 individuals underwent two 31P-MRS testing sessions to assess the test-retest reproducibility of the method. Overall, the in vivo and in vitro methods were well-correlated (r = 0.66 –0.72) and showed good agreement by Bland Altman plots. Excellent reproducibility was observed for the PCr recovery rate constant (CV = 4.6%, ICC = 0.85) and calculated oxidative capacity (CV = 3.4%, ICC = 0.83). These results indicate that 31P-MRS corresponds well with gold-standard in vitro measurements and is highly reproducible.
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影响因子：
3.3
作者：
Kent-Braun, JA;Ng, AV
通讯作者：
Ng, AV
DOI：
10.1073/pnas.82.24.8384
发表时间：
1985-12-01
影响因子：
11.1
作者：
CHANCE, B;LEIGH, JS;SMITH, D
通讯作者：
SMITH, D
DOI：
10.1007/s10334-006-0060-0
发表时间：
2006-12-01
影响因子：
2.3
作者：
Praet, S. F. E.;De Feyter, H. M. M.;Prompers, J. J.
通讯作者：
Prompers, J. J.
影响因子：
3.3
作者：
Lanza, IR;Befroy, DE;Kent-Braun, JA
通讯作者：
Kent-Braun, JA
影响因子：
168.9
作者：
Pulkes, T;Sweeney, MG;Hanna, MG
通讯作者：
Hanna, MG