Different potato virus Y strains frequently co-localize in single epidermal leaf cells and in the aphid stylet.
Different potato virus Y strains frequently co-localize in single epidermal leaf cells and in the aphid stylet.
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不同的马铃薯 Y 病毒株经常共存于单个表皮叶细胞和蚜虫管心中。
DOI：
10.1099/jgv.0.001576
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Mondal S
中科院分区：
文献类型：
--
作者：
Mondal S
单头蚜虫可以同时或连续获得和传播多个马铃薯Y病毒(PVY)菌株。多个PVY菌株经常在同一块田地中被发现，偶尔在同一植物中被发现，但对PVY菌株如何在植物中或在蚜虫小口针中相互作用知之甚少。用免疫染色和共聚焦显微镜观察了PVY菌株(PVYO和PVYNTN或PVYO和PVYN)混合菌株在‘SamSun NN’烟草和‘Goldrush’马铃薯叶片细胞中的时空动态。在获得病毒后，还检查了蚜虫刺针中的病毒结合和定位。这两个菌株都系统感染烟草，并共同定位于所有被检查的叶片的细胞中；然而，每种病毒的相对数量随着时间的推移而变化。在烟草侵染早期，当观察到花叶症状时，虽然在一些细胞中检测到PVYNTN，但PVYO占主导地位。随着感染的进展和静脉坏死的发展，PVYNTN是普遍存在的。PVYO和PVYN同时存在于马铃薯叶片的表皮细胞中，大多数细胞同时感染PVYO和PVYN。此外，在从感染了菌株混合物的植物上获得病毒后，可以检测到两个菌株与蚜虫刺针的远端结合。这些数据与两个紧密相关的马铃薯Y病毒在空间上分离的传统观念相反，表明PVY株系之间明显的非拮抗相互作用，这可能有助于解释近年来在马铃薯上发现的大量重组PVY株系。
Single aphids can simultaneously or sequentially acquire and transmit multiple potato virus Y (PVY) strains. Multiple PVY strains are often found in the same field and occasionally within the same plant, but little is known about how PVY strains interact in plants or in aphid stylets. Immuno-staining and confocal microscopy were used to examine the spatial and temporal dynamics of PVY strain mixtures (PVYOand PVYNTNor PVYOand PVYN) in epidermal leaf cells of ‘Samsun NN’ tobacco and ‘Goldrush’ potato. Virus binding and localization was also examined in aphid stylets following acquisition. Both strains systemically infected tobacco and co-localized in cells of all leaves examined; however, the relative amounts of each virus changed over time. Early in the tobacco infection, when mosaic symptoms were observed, PVYOdominated the infection although PVYNTNwas detected in some cells. As the infection progressed and vein necrosis developed, PVYNTNwas prevalent. Co-localization of PVYOand PVYNwas also observed in epidermal cells of potato leaves with most cells infected with both viruses. Furthermore, two strains could be detected binding to the distal end of aphid stylets following virus acquisition from a plant infected with a strain mixture. These data are in contrast with the traditional belief of spatial separation of two closely related potyviruses and suggest apparent non-antagonistic interaction between PVY strains that could help explain the multitude of emerging recombinant PVY strains discovered in potato in recent years.
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马铃薯Y病毒（PVY）在马铃薯植株维管系统中的运动
影响因子：
1.8
作者：
B. Dupuis
通讯作者：
B. Dupuis
矿物油与杀虫剂联合控制蚜虫病毒载体以减少种薯（Solanum tuberosum）马铃薯病毒 Y 感染的功效
DOI：
10.1080/09064710.2011.583936
发表时间：
2012
期刊：
Acta Agriculturae Scandinavica, Section B — Soil & Plant Science
影响因子：
--
作者：
L. M. Hansen;S. Nielsen
通讯作者：
S. Nielsen
桃蝇马铃薯Y病毒属密切相关株的株系特异性及同时传播
影响因子：
2.2
作者：
R. Srinivasan;Darren G. Hall;Felix A Cervantes;J. M. Álvarez;J. Whitworth
通讯作者：
J. Whitworth
哥伦比亚马铃薯黄脉病毒和马铃薯Y病毒对马铃薯作物的自然共感染
影响因子：
2.7
作者：
Angela Villamil;W. Cuellar;M. Guzmán
通讯作者：
M. Guzmán
影响因子：
2.6
作者：
Verbeek, M.;Piron, P. G. M.;van der Vlugt, R. A. A.
通讯作者：
van der Vlugt, R. A. A.