Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography
Assessment of cervical demineralization induced by Streptococcus mutans using swept-source optical coherence tomography
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使用扫源光学相干断层扫描评估变形链球菌引起的宫颈脱矿
DOI：
10.1117/1.jmi.3.1.014504
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发表时间：
2016
影响因子：
2.4
通讯作者：
Tagami J
中科院分区：
文献类型：
--
作者：
Tezuka H;Shimada Y;Matin K;Ikeda M;Sadr A;Sumi Y;Tagami J
由于牙龈退缩而暴露的牙根表面会受到生物膜停滞的影响，从而导致龋齿的形成。采用扫描光源光学相干断层扫描（SS-OCT）评价致龋生物膜引起的牙颈部釉质和牙本质脱矿。牙骨质釉质交界处（CEJ）部分提取的人牙进行脱矿1，2，或3周。应用口腔生物膜反应器将变形链球菌悬液用于形成致龋生物膜。孵育后，通过SS-OCT观察脱矿作用。为了分析SS-OCT信号，测量信号曲线的曲线下面积（AUC）值。在95%置信水平下进行统计分析。颈段脱矿在SS-OCT上显示为亮区，牙本质脱矿深度明显大于牙釉质脱矿深度（）。在脱矿后，CEJ附近的釉质表现出AUC比其他釉质区域显著增加。在SS-OCT中还观察到牙本质-釉质连接处的沿着间隙。SS-OCT能够监测由致龋生物膜引起的宫颈脱矿，被认为是诊断宫颈脱矿的一种有前途的方法。
Exposed root surfaces due to gingival recession are subject to biofilm stagnation that can result in caries formation. Cervical enamel and dentin demineralization induced by a cariogenic biofilm was evaluated using swept-source optical coherence tomography (SS-OCT). The cementoenamel junction (CEJ) sections of extracted human teeth were subjected to demineralization for 1, 2, or 3 weeks. A suspension ofStreptococcus mutanswas applied to form a cariogenic biofilm using an oral biofilm reactor. After incubation, demineralization was observed by SS-OCT. For the analysis of SS-OCT signal, the value of the area under the curve (AUC) of the signal profile was measured. Statistical analyses were performed with 95% level of confidence. Cervical demineralization was displayed as a bright zone in SS-OCT. The demineralization depth of dentin was significantly deeper than that of enamel (). Enamel near the CEJ demonstrated a significant increase of AUC over the other enamel region after the demineralization. The gaps along the dentinoenamel junction were additionally observed in SS-OCT. SS-OCT was capable of monitoring the cervical demineralization induced by a cariogenic biofilm and is considered to be a promising modality for the diagnosis of cervical demineralization.
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影响因子：
5
作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
--
发表时间：
2010
期刊：
影响因子：
--
作者：
Makishi P;Shimada Y;Sadr A;Sumi Y;Tagami J
通讯作者：
Tagami J